A new method for the encapsulation of mammalian cells
| ISSN: |
1573-0778
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|---|---|
| Keywords: |
cell culture ; cellulose sulphate ; encapsulation ; monoclonal antibodies ; poly-dimethyl-diallyl-ammoniumchloride
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
Process Engineering, Biotechnology, Nutrition Technology
|
| Notes: |
Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798297440935215104 |
|---|---|
| autor | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| autorsonst | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| book_url | http://dx.doi.org/10.1007/BF00350918 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM193663791 |
| issn | 1573-0778 |
| journal_name | Cytotechnology |
| materialart | 1 |
| notes | Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process. |
| package_name | Springer |
| publikationsjahr_anzeige | 1991 |
| publikationsjahr_facette | 1991 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1991 |
| publisher | Springer |
| reference | 7 (1991), S. 121-130 |
| schlagwort | cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride |
| search_space | articles |
| shingle_author_1 | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| shingle_author_2 | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| shingle_author_3 | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| shingle_author_4 | Merten, O. W. Dautzenberg, H. Palfi, G. E. |
| shingle_catch_all_1 | Merten, O. W. Dautzenberg, H. Palfi, G. E. A new method for the encapsulation of mammalian cells cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process. 1573-0778 15730778 Springer |
| shingle_catch_all_2 | Merten, O. W. Dautzenberg, H. Palfi, G. E. A new method for the encapsulation of mammalian cells cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process. 1573-0778 15730778 Springer |
| shingle_catch_all_3 | Merten, O. W. Dautzenberg, H. Palfi, G. E. A new method for the encapsulation of mammalian cells cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process. 1573-0778 15730778 Springer |
| shingle_catch_all_4 | Merten, O. W. Dautzenberg, H. Palfi, G. E. A new method for the encapsulation of mammalian cells cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride cell culture cellulose sulphate encapsulation monoclonal antibodies poly-dimethyl-diallyl-ammoniumchloride Abstract A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammoniumchloride (PDMDAAC). When CS droplets fell into the precipitation bath consisting of a 2% solution of PDMDAAC, immediately a membrane at the interphase was built up. The influences of varying encapsulation process parameters on capsule characteristics, cell growth, and monoclonal antibody production were tested. This new method showed advantages when compared to other methods mainly due to time simplicity of the whole process. 1573-0778 15730778 Springer |
| shingle_title_1 | A new method for the encapsulation of mammalian cells |
| shingle_title_2 | A new method for the encapsulation of mammalian cells |
| shingle_title_3 | A new method for the encapsulation of mammalian cells |
| shingle_title_4 | A new method for the encapsulation of mammalian cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T10:08:02.540Z |
| titel | A new method for the encapsulation of mammalian cells |
| titel_suche | A new method for the encapsulation of mammalian cells |
| topic | W WW-YZ ZM |
| uid | nat_lic_papers_NLM193663791 |