Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome

Publication Date:
2018-03-09
Publisher:
BMJ Publishing Group
Print ISSN:
0017-5749
Electronic ISSN:
1468-3288
Topics:
Medicine
Keywords:
Open access, Editor's choice
Published by:
http://www.bmj.com/
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autor Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
beschreibung Objective Faecal microbiota transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection (CDI). Studies have shown bacterial colonisation after FMT, but data on viral alterations in CDI are scarce. We investigated enteric virome alterations in CDI and the association between viral transfer and clinical outcome in patients with CDI. Design Ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on stool samples from 24 subjects with CDI and 20 healthy controls. We longitudinally assessed the virome and bacterial microbiome changes in nine CDI subjects treated with FMT and five treated with vancomycin. Enteric virome alterations were assessed in association with treatment response. Results Subjects with CDI demonstrated a significantly higher abundance of bacteriophage Caudovirales and a lower Caudovirales diversity, richness and evenness compared with healthy household controls. Significant correlations were observed between bacterial families Proteobacteria , Actinobacteria and Caudovirales taxa in CDI. FMT treatment resulted in a significant decrease in the abundance of Caudovirales in CDI. Cure after FMT was observed when donor-derived Caudovirales contigs occupied a larger fraction of the enteric virome in the recipients (p=0.024). In treatment responders, FMT was associated with alterations in the virome and the bacterial microbiome, while vancomycin treatment led to alterations in the bacterial community alone. Conclusions In a preliminary study, CDI is characterised by enteric virome dysbiosis. Treatment response in FMT was associated with a high colonisation level of donor-derived Caudovirales taxa in the recipient. Caudovirales bacteriophages may play a role in the efficacy of FMT in CDI. Trial registration number NCT02570477
citation_standardnr 6199104
datenlieferant ipn_articles
feed_id 3103
feed_publisher BMJ Publishing Group
feed_publisher_url http://www.bmj.com/
insertion_date 2018-03-09
journaleissn 1468-3288
journalissn 0017-5749
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher BMJ Publishing Group
quelle Gut
relation http://gut.bmj.com/cgi/content/short/67/4/634?rss=1
schlagwort Open access, Editor's choice
search_space articles
shingle_author_1 Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
shingle_author_2 Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
shingle_author_3 Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
shingle_author_4 Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
shingle_catch_all_1 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
Open access, Editor's choice
Objective Faecal microbiota transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection (CDI). Studies have shown bacterial colonisation after FMT, but data on viral alterations in CDI are scarce. We investigated enteric virome alterations in CDI and the association between viral transfer and clinical outcome in patients with CDI. Design Ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on stool samples from 24 subjects with CDI and 20 healthy controls. We longitudinally assessed the virome and bacterial microbiome changes in nine CDI subjects treated with FMT and five treated with vancomycin. Enteric virome alterations were assessed in association with treatment response. Results Subjects with CDI demonstrated a significantly higher abundance of bacteriophage Caudovirales and a lower Caudovirales diversity, richness and evenness compared with healthy household controls. Significant correlations were observed between bacterial families Proteobacteria , Actinobacteria and Caudovirales taxa in CDI. FMT treatment resulted in a significant decrease in the abundance of Caudovirales in CDI. Cure after FMT was observed when donor-derived Caudovirales contigs occupied a larger fraction of the enteric virome in the recipients (p=0.024). In treatment responders, FMT was associated with alterations in the virome and the bacterial microbiome, while vancomycin treatment led to alterations in the bacterial community alone. Conclusions In a preliminary study, CDI is characterised by enteric virome dysbiosis. Treatment response in FMT was associated with a high colonisation level of donor-derived Caudovirales taxa in the recipient. Caudovirales bacteriophages may play a role in the efficacy of FMT in CDI. Trial registration number NCT02570477
Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
BMJ Publishing Group
0017-5749
00175749
1468-3288
14683288
shingle_catch_all_2 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
Open access, Editor's choice
Objective Faecal microbiota transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection (CDI). Studies have shown bacterial colonisation after FMT, but data on viral alterations in CDI are scarce. We investigated enteric virome alterations in CDI and the association between viral transfer and clinical outcome in patients with CDI. Design Ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on stool samples from 24 subjects with CDI and 20 healthy controls. We longitudinally assessed the virome and bacterial microbiome changes in nine CDI subjects treated with FMT and five treated with vancomycin. Enteric virome alterations were assessed in association with treatment response. Results Subjects with CDI demonstrated a significantly higher abundance of bacteriophage Caudovirales and a lower Caudovirales diversity, richness and evenness compared with healthy household controls. Significant correlations were observed between bacterial families Proteobacteria , Actinobacteria and Caudovirales taxa in CDI. FMT treatment resulted in a significant decrease in the abundance of Caudovirales in CDI. Cure after FMT was observed when donor-derived Caudovirales contigs occupied a larger fraction of the enteric virome in the recipients (p=0.024). In treatment responders, FMT was associated with alterations in the virome and the bacterial microbiome, while vancomycin treatment led to alterations in the bacterial community alone. Conclusions In a preliminary study, CDI is characterised by enteric virome dysbiosis. Treatment response in FMT was associated with a high colonisation level of donor-derived Caudovirales taxa in the recipient. Caudovirales bacteriophages may play a role in the efficacy of FMT in CDI. Trial registration number NCT02570477
Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
BMJ Publishing Group
0017-5749
00175749
1468-3288
14683288
shingle_catch_all_3 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
Open access, Editor's choice
Objective Faecal microbiota transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection (CDI). Studies have shown bacterial colonisation after FMT, but data on viral alterations in CDI are scarce. We investigated enteric virome alterations in CDI and the association between viral transfer and clinical outcome in patients with CDI. Design Ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on stool samples from 24 subjects with CDI and 20 healthy controls. We longitudinally assessed the virome and bacterial microbiome changes in nine CDI subjects treated with FMT and five treated with vancomycin. Enteric virome alterations were assessed in association with treatment response. Results Subjects with CDI demonstrated a significantly higher abundance of bacteriophage Caudovirales and a lower Caudovirales diversity, richness and evenness compared with healthy household controls. Significant correlations were observed between bacterial families Proteobacteria , Actinobacteria and Caudovirales taxa in CDI. FMT treatment resulted in a significant decrease in the abundance of Caudovirales in CDI. Cure after FMT was observed when donor-derived Caudovirales contigs occupied a larger fraction of the enteric virome in the recipients (p=0.024). In treatment responders, FMT was associated with alterations in the virome and the bacterial microbiome, while vancomycin treatment led to alterations in the bacterial community alone. Conclusions In a preliminary study, CDI is characterised by enteric virome dysbiosis. Treatment response in FMT was associated with a high colonisation level of donor-derived Caudovirales taxa in the recipient. Caudovirales bacteriophages may play a role in the efficacy of FMT in CDI. Trial registration number NCT02570477
Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
BMJ Publishing Group
0017-5749
00175749
1468-3288
14683288
shingle_catch_all_4 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
Open access, Editor's choice
Objective Faecal microbiota transplantation (FMT) is effective for the treatment of recurrent Clostridium difficile infection (CDI). Studies have shown bacterial colonisation after FMT, but data on viral alterations in CDI are scarce. We investigated enteric virome alterations in CDI and the association between viral transfer and clinical outcome in patients with CDI. Design Ultra-deep metagenomic sequencing of virus-like particle preparations and bacterial 16S rRNA sequencing were performed on stool samples from 24 subjects with CDI and 20 healthy controls. We longitudinally assessed the virome and bacterial microbiome changes in nine CDI subjects treated with FMT and five treated with vancomycin. Enteric virome alterations were assessed in association with treatment response. Results Subjects with CDI demonstrated a significantly higher abundance of bacteriophage Caudovirales and a lower Caudovirales diversity, richness and evenness compared with healthy household controls. Significant correlations were observed between bacterial families Proteobacteria , Actinobacteria and Caudovirales taxa in CDI. FMT treatment resulted in a significant decrease in the abundance of Caudovirales in CDI. Cure after FMT was observed when donor-derived Caudovirales contigs occupied a larger fraction of the enteric virome in the recipients (p=0.024). In treatment responders, FMT was associated with alterations in the virome and the bacterial microbiome, while vancomycin treatment led to alterations in the bacterial community alone. Conclusions In a preliminary study, CDI is characterised by enteric virome dysbiosis. Treatment response in FMT was associated with a high colonisation level of donor-derived Caudovirales taxa in the recipient. Caudovirales bacteriophages may play a role in the efficacy of FMT in CDI. Trial registration number NCT02570477
Zuo, T., Wong, S. H., Lam, K., Lui, R., Cheung, K., Tang, W., Ching, J. Y. L., Chan, P. K. S., Chan, M. C. W., Wu, J. C. Y., Chan, F. K. L., Yu, J., Sung, J. J. Y., Ng, S. C.
BMJ Publishing Group
0017-5749
00175749
1468-3288
14683288
shingle_title_1 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
shingle_title_2 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
shingle_title_3 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
shingle_title_4 Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
timestamp 2025-06-30T23:33:24.095Z
titel Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
titel_suche Bacteriophage transfer during faecal microbiota transplantation in Clostridium difficile infection is associated with treatment outcome
topic WW-YZ
uid ipn_articles_6199104