Search Results - (Author, Cooperation:P. McCourt)
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1Latest Papers from Table of Contents or Articles in PressS. Toh ; D. Holbrook-Smith ; P. J. Stogios ; O. Onopriyenko ; S. Lumba ; Y. Tsuchiya ; A. Savchenko ; P. McCourt
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-10-10Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Amino Acid Sequence ; Arabidopsis/genetics/metabolism ; Catalytic Domain ; Germination/drug effects ; Heterocyclic Compounds, 3-Ring/*metabolism/pharmacology ; Lactones/*metabolism/pharmacology ; Molecular Sequence Data ; Phylogeny ; Plant Growth Regulators/*metabolism/pharmacology ; Plant Proteins/*chemistry/classification/genetics ; Protein Structure, Secondary ; Receptors, Cell Surface/*chemistry/classification/genetics ; Seeds/genetics/growth & development/metabolism ; Striga/genetics/growth & development/*metabolism ; Structure-Activity RelationshipPublished by: -
2Latest Papers from Table of Contents or Articles in PressY. Tsuchiya ; M. Yoshimura ; Y. Sato ; K. Kuwata ; S. Toh ; D. Holbrook-Smith ; H. Zhang ; P. McCourt ; K. Itami ; T. Kinoshita ; S. Hagihara
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-08-22Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Fluoresceins/chemistry/metabolism ; Fluorescence ; Fluorescent Dyes/chemistry/metabolism ; *Germination ; Hydrolases/metabolism ; Hydrolysis ; Lactones/*metabolism ; Molecular Imaging/methods ; Molecular Sequence Data ; Plant Growth Regulators/*metabolism ; Plant Proteins/genetics/*metabolism ; Receptors, Cell Surface/genetics/*metabolism ; Seeds/*growth & development/metabolism ; Signal Transduction ; Striga/*growth & development/metabolismPublished by: -
3Latest Papers from Table of Contents or Articles in PressUraguchi, D., Kuwata, K., Hijikata, Y., Yamaguchi, R., Imaizumi, H., AM, S., Rakers, C., Mori, N., Akiyama, K., Irle, S., McCourt, P., Kinoshita, T., Ooi, T., Tsuchiya, Y.
American Association for the Advancement of Science (AAAS)
Published 2018Staff ViewPublication Date: 2018-12-14Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyGeosciencesComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: BotanyPublished by: -
4Articles: DFG German National LicensesStaff View
ISSN: 1573-2665Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The low abundance lysosomal enzymeN-acetylgalactosamine 4-sulphatase (4-sulphatase) has been quantified using a microimmunopurification step and a monoclonal-based ELISA detection system. The assay is similar in principle to a two-site ELISA but uses a single monoclonal antibody against one epitope to bind 4-sulphatase in two separate assay steps. The sensitivity of this assay is sufficient to allow the quantification of 4-sulphatase in human cultured skin fibroblasts derived from normal controls and patients deficient in 4-sulphatase activity (mucopolysaccharidosis type VI or Maroteaux-Lamy syndrome). The results obtained suggest a range of mucopolysaccharidosis type VI or 4-sulphatase deficient mutants, from those expressing little or no quantifiable 4-sulphatase protein to those examples with quantifiable levels of 4-sulphatase protein which is enzymically inactive. Phenotypic variability in patients with a 4-sulphatase deficiency may therefore be partially attributed to a range of protein expressions. The method should allow the determination of 4-sulphatase specific activity in mucopolysaccharidosis type VI patients.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1573-2665Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary A method combining immune capture and enzyme detection by fluorochemistry has been developed for the diagnostic assay of N-acetylgalactosamine-4-sulphatase (4-sulphatase). The procedure uses a monoclonal antibody 4-S 4.1 to immunoadsorb 4-sulphatase specifically from complex protein samples containing other sulphatases, and 4-methylumbelliferyl sulphate to detect captured 4-sulphatase. The assay provides an accurate and simple method for the diagnosis of Maroteaux-Lamy syndrome (Mucopolysaccharidosis type VI).Type of Medium: Electronic ResourceURL: