Search Results - (Author, Cooperation:P. Lashermes)
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1Latest Papers from Table of Contents or Articles in PressF. Denoeud ; L. Carretero-Paulet ; A. Dereeper ; G. Droc ; R. Guyot ; M. Pietrella ; C. Zheng ; A. Alberti ; F. Anthony ; G. Aprea ; J. M. Aury ; P. Bento ; M. Bernard ; S. Bocs ; C. Campa ; A. Cenci ; M. C. Combes ; D. Crouzillat ; C. Da Silva ; L. Daddiego ; F. De Bellis ; S. Dussert ; O. Garsmeur ; T. Gayraud ; V. Guignon ; K. Jahn ; V. Jamilloux ; T. Joet ; K. Labadie ; T. Lan ; J. Leclercq ; M. Lepelley ; T. Leroy ; L. T. Li ; P. Librado ; L. Lopez ; A. Munoz ; B. Noel ; A. Pallavicini ; G. Perrotta ; V. Poncet ; D. Pot ; Priyono ; M. Rigoreau ; M. Rouard ; J. Rozas ; C. Tranchant-Dubreuil ; R. VanBuren ; Q. Zhang ; A. C. Andrade ; X. Argout ; B. Bertrand ; A. de Kochko ; G. Graziosi ; R. J. Henry ; Jayarama ; R. Ming ; C. Nagai ; S. Rounsley ; D. Sankoff ; G. Giuliano ; V. A. Albert ; P. Wincker ; P. Lashermes
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-06Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Caffeine/biosynthesis/*genetics ; Coffea/classification/*genetics ; *Evolution, Molecular ; *Genome, Plant ; Methyltransferases/genetics/*physiology ; Phylogeny ; Plant Proteins/genetics/*physiologyPublished by: -
2Articles: DFG German National LicensesAnthony, F. ; Quiros, O. ; Topart, P. ; Bertrand, B. ; Lashermes, P.
Oxford, UK : Blackwell Publishing Ltd
Published 2002Staff ViewISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Microsatellites or simple sequence repeats (SSR) were used to assess polymorphism among 16 Coffea arabica and four Coffea canephora accessions, and to identify DNA introgression fragments from C. canephora in four C. arabica lines. Thirty-one primer pairs allowed for the identification of 92 polymorphic alleles distributed over 37 loci. The C. arabica accessions derived from the genetic bases ‘Typica’ and ‘Bourbon’ were grouped separately according to their genetic origin. Two genotypes derived from a spontaneous hybrid (C arabica×C. canephora) were classified with the C. canephora accessions from Central Africa. Coffea canephora from West Africa were separated from the other accessions studied. Four alleles related to introgression (i.e. present in C. canephora and introgressed lines, and absent in C. arabica) were identified. The SSR markers were used successfully for characterization of a particular cultivar (‘Veranero’) from Costa Rica, which is known for its late maturity.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesRival, A. ; Bertrand, L. ; Beulé, T. ; Combes, M.C. ; Trouslot, P. ; Lashermes, P.
Oxford, UK : Blackwell Publishing Ltd
Published 1998Staff ViewISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Random amplified polymorphic DNA (RAPD) analysis using arbitrary 10-mer oligonucleotide primers was employed in order to investigate the genetic fidelity of somatic embryogenesis-derived regenerants of oil palm (Elaeis guineensis Jacq). Clonal palms bearing the ‘mantled’ phenotype were identified in the field and the ability of RAPD markers to distinguish these variants from palms of the normal type was assessed. Of the 387 arbitrary primers used, 259 (67%) were successfully used to amplify oil palm DNA genomic fragments with consistently reproducible banding. Of these 387 primers, 73 (19%) primers enabled the identification of polymorphism between clones. No intraclonal variability and no differences between mother palms and regenerants could be identified using the total number of markers scored (8900). Twenty-four of these 73 primers were chosen for use in a larger experiment aimed at comparing, first, the mother palm genome with that of its clonal offspring and, second, true-to-type and variant regenerants. Thus, the regeneration protocol based on somatic embryogenesis set up for oil palm clonal propagation does not induce any gross genetic changes. The results obtained revealed however, that the RAPD approach is not suitable for the detection of the ‘mantled’ variant phenotype. The use of RAPD markers for the detection of somaclonal variation in oil palm is discussed.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Fifty-five doubled haploids (DH) of Coffea canephora were crossed with either heterozygous genotypes or DH in order to study their combining ability. Three agronomic trials were established. Marked hybrid vigour was observed for all characters analyzed including yield. Large differences were evident among top-crosses involving different DH produced from the same parental clone reflecting the high level of heterozygosity of clones. Factorial mating design analysis indicated that all genetic variance was attributable to additive effects in estimates of yield as well as plant height and leaf characteristics. The general combining ability variance component was also predominant for stem girth and susceptibility to leaf rust, although effects due to interaction were detected. Some hybrid combinations had yield comparable to standard clonal varieties. The implications of such results for breeding of Coffea canephora are discussed. Particularly, the development of F1 hybrid varieties is envisaged.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesNoir, S. ; Anthony, F. ; Bertrand, B. ; Combes, M.-C. ; Lashermes, P.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-3059Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Among the most damaging root-knot nematode species, Meloidogyne exigua is especially common in Latin America and constitutes a major agronomic constraint in all major coffee-growing (Coffea arabica) areas. Growing nematode-resistant coffee represents the most promising option for control of the pest. The present study aimed to determine the mode of inheritance of the M. exigua resistance transferred into C. arabica from a related species, Coffea canephora, and to identify associated molecular markers. Segregation data analysis of F2 progeny derived from a cross between the resistant introgression line T5296 and the susceptible accession Et6 showed that the resistance to M. exigua is controlled by a simply inherited major gene (designated the Mex-1 locus). The gall index distribution exhibited by the F2 individuals suggested incomplete dominant expression. Fourteen AFLP markers were found associated with the resistance to M. exigua and a localized genetic map of the chromosome segment carrying Mex-1 was constructed. Furthermore, the association of the identified AFLP markers with Mex-1 was confirmed by analysis of a set of genotypes involving 28 introgression Arabica lines either resistant or susceptible to M. exigua in field conditions. These results represent an important starting point to enhance backcross breeding programmes and to perform an early selection of resistant seedlings.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1365-3059Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Breeding for resistance to root-knot nematode Meloidogyne exigua in coffee may help in controlling this nematode, which causes substantial harvest losses throughout Latin America. Accessions of Coffea arabica, C. canephora and lines derived from the Timor Hybrid (wild C. arabica × C. canephora interspecific hybrid) were tested in a greenhouse for resistance to gall formation by counting individual galls on the roots of plants inoculated with second-stage juveniles of a M. exigua population from Costa Rica. The level of introgression from C. canephora was also assessed on 28 resistant and susceptible genotypes in a molecular study with amplified fragment length polymorphism (AFLP) markers. The frequency of resistant plants was very low or even nonexistent in C. arabica and very high in C. canephora with 〉 78% immune plants and 100% resistant plants. Several lines derived from the interspecific hybrid revealed a high level of resistance similar to that observed in the parent C. canephora species. A molecular study of Timor Hybrid-derived lines revealed high variability for the number of markers present in the study genotypes. A larger number of introgression markers was found in the group of resistant genotypes than in the susceptible genotypes. However, there were also lines with little introgression that had retained resistance. Based on genetic distances calculated from these markers, two groups of lines were revealed: those derived from Timor Hybrid CIFC1343, and those derived from Timor Hybrids CIFC832/1 and CIFC832/2, which may indicate the existence of different resistance genes in the two groups. The segregations observed in F1 and F2 progeny may be explained by at least a dominant gene. The lines derived from the Timor Hybrid are a worthwhile source of resistance to M. exigua that can be exploited to improve C. arabica with the help of molecular-assisted selection.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesLashermes, P. ; Andrzejewski, S. ; Bertrand, B. ; Combes, M. C. ; Dussert, S. ; Graziosi, G. ; Trouslot, P. ; Anthony, F.
Springer
Published 2000Staff ViewISSN: 1432-2242Keywords: Key words Coffea arabica ; Introgression ; Genetic diversity ; Alien gene transfer ; AFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Nineteen arabica coffee introgression lines (BC1F4) and two accessions derived from a spontaneous interspecific cross (i.e. Timor Hybrid) between Coffea arabica (2n=4x=44) and C. canephora (2n=2x=22) were analysed for the introgression of C. canephora genetic material. The Timor Hybrid-derived genotypes were evaluated by AFLP, using 42 different primer combinations, and compared to 23 accessions of C. arabica and 8 accessions of C. canephora. A total of 1062 polymorphic fragments were scored among the 52 accessions analysed. One hundred and seventy-eight markers consisting of 109 additional bands (i.e. introgressed markers) and 69 missing bands distinguished the group composed of the Timor Hybrid-derived genotypes from the accessions of C. arabica. AFLP therefore seemed to be an extremely efficient technique for DNA marker generation in coffee as well as for the detection of introgression in C. arabica. The genetic diversity observed in the Timor Hybrid-derived genotypes appeared to be approximately double that in C. arabica. Although representing only a small proportion of the genetic diversity available in C. canephora, the Timor Hybrid obviously constitutes a considerable source of genetic diversity for arabica breeding. Analysis of genetic relationships among the Timor Hybrid-derived genotypes suggested that introgression was not restricted to chromosome substitution but also involved chromosome recombinations. Furthermore, the Timor Hybrid-derived genotypes varied considerably in the number of AFLP markers attributable to introgression. In this way, the introgressed markers identified in the analysed arabica coffee introgressed genotypes were estimated to represent from 9% to 29% of the C. canephora genome. Nevertheless, the amount of alien genetic material in the introgression arabica lines remains substantial and should justify the development of adapted breeding strategies.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Key wordsCoffea canephora ; Self-incompatibility ; Gametophytic system ; S-locus ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea canephora was established using both phenotypic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogenous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self-incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following incompatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Coffea canephora ; Self-incompatibility ; Gametophytic system ; S-locus ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Cross-compatibility behaviour of doubled haploid (DH) and hybrid genotypes of Coffea camphora was established using both phenotypic bioassay and in situ seed-set examination. The availability of DHs provided the opportunity of working with genetically homogenous pollen and female parents. The aniline blue fluorescence (ABF) method was applied to detect callose accumulation in pollen and pistil. Clear cross-compatibility/incompatibility situations were observed and confirmed by in situ seed-set analysis. Cross-compatibility analysis of hybrid combinations involving different DHs corroborated the crossing behaviour observed at the DH level. Expression of the self-incompatibility system did not appear to be affected by the low vigour of the DH. The crossing-behaviour distribution observed within DHs derived from clone IF200 confirmed that self-incompatibility in C. canephora is a gametophytic self-incompatibility system controlled by a single locus (S-locus). Reduced seed-set developments following incompatible crosses may indicate the occurrence of pseudo-incompatibility. Molecular marker linkage analysis showed that the S-locus is associated with an RFLP marker on linkage group 9. The availability of a linked DNA marker should facilitate the genetic analysis of self-incompatibility in relation to coffee breeding programmes.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Key words Coffea ; Coffee-tree ; Internal transcribed spacer region ; Nuclear ribosomal DNA ; Molecular phylogenySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Phylogenetic relationships of Coffea species were estimated from the sequences of the internal transcribed spacer (ITS 2) region of nuclear ribosomal DNA. The ITS 2 region of 37 accessions belonging to 26 Coffea taxa and to three Psilanthus species was directly sequenced from polymerase chain reaction (PCR)-amplified DNA fragments. The level of variation was high enough to make the ITS 2 a useful tool for phylogenetic reconstruction. However, an unusual level of intraspecific variation was observed leading to some difficulty in interpreting rDNA sequence divergences. Sequences were analysed using Wagner parsimony as well as the neighbour-joining distance method. Coffea taxa were divided into several major groups which present a strong geographical correspondence (i.e. Madagascar, East Africa, Central Africa and West Africa). This organisation is well supported by cytogenetic evidence. On the other hand, the results were in contradiction with the present classification of coffee-tree taxa into two genera, namely Coffea and Psilanthus. Furthermore, additivity of parental rDNA types was not observed in the allotetraploid species C. arabica.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Zea mays ; Haploid induction ; Gynogenesis ; Genetics ; Inducer lineSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary The effect of genotype on maternal haploid plant production in maize was studied. The frequency of gynogenetic plants when “Stock 6” was used as pollinator varied according to the female parent genotype. No simple relation was observed between genotypic aptitudes for gynogenetic and androgenetic development, which occured after pollination of “W23” plant carrying the “indeterminate gametophyte” gene. Furthermore, the population NS, a favorably responsive genotype to anther culture, does not exhibit exceptional ability for in vivo gynogenesis. The effect of inbreeding and the influence of maternal haploid origin suggest that specific genes control maternal haploid initiation and development. However, gynogenetic development is not limited to a particular genotype. The frequency of maternal haploids may be increased by using specific pollen parents. Attempts were made to select for a high haploidyinducing trait and the present study reports the successful development of lines that can be utilized as pollen parents to induce haploids for experimental purposes and breeding programmes. When an inbred line “WS14”, derived from the cross W23 x Stock 6, was used as pollen parent, 2%–5% maternal haploids were obtained according to the female parent genotype. A high haploidy-inducing potential is a heritable trait and may be controlled by a limited number of genes. Genetic determination of the haploidy-inducing character was examined in relation to the efficiency of the selecting method and the mechanisms involved in the origin of maternal haploids.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Zea mays ; Haploid ; Gynogenesis ; Gametophytic selection ; Genetic markersSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary Experiments were conducted to investigate whether selection occurs during the processes involved in the production of doubled haploids. Haploid plants produced from two hybrids, each heterozygous for isozyme markers, were subjected to genetic analysis. The distributions of doubled haploid lines and pedigree lines derived from the hybrid C123 x Oh7 were compared with regard to agronomic character. The results suggest that the populations of haploid plants obtained by in vivo gynogenesis represent a random gametic array. Thus, in order to introduce haploid plants into breeding programmes in maize, maternal haploidy seems to be a very attractive method.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Coffee ; RAPD ; RFLP ; Linkage map ; Doubled haploidsSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A linkage map for coffee (Coffea canephora P.) totalling 1402 cM has been developed on the basis of a population of doubled haploids. Both RFLP markers and PCR-based markers (RAPD) were used to construct 15 linkage groups. Coffee genomic and cDNA clones provided the source of the probes. In total, 47 RFLP and 100 RAPD loci have been placed on the linkage map. A rather low DNA polymorphism rate (18% for RFLP markers and 29% for RAPD primers) was detected. Only 81% of RAPD markers and 85% of RFLP markers fit an expected 1∶1 ratio (P〈0.01). The availability of a molecular linkage map has many implications for the future development of the genetics and breeding of this commercially important crop species.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesLashermes, P. ; Cros, J. ; Combes, M. C. ; Trouslot, P. ; Anthony, F. ; Hamon, S. ; Charrier, A.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Chloroplast DNA ; Coffea ; Inheritance ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract CpDNA variation among 52 tree samples belonging to 25 different taxa of Coffea and two species of Psilanthus was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region. Twelve variable characters were distinguished allowing the identification of 12 different plastomes. The low sequence divergence observed might suggest that Coffea is a young genus. The results were in contradiction with the present classification into two genera. Additionally, cpDNA inheritance was studied in interspecific hybrids between C. arabica and C. canephora, and in an intraspecific progeny of C. canephora, using PCR-based markers. Both studies showed exclusively maternal inheritance of cpDNA.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: Key wordsCoffee ; RAPD ; RFLP ; Linkage map ; Doubled haploidsSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A linkage map for coffee (Coffea canephora P.) totalling 1402 cM has been developed on the basis of a population of doubled haploids. Both RFLP markers and PCR-based markers (RAPD) were used to construct 15 linkage groups. Coffee genomic and cDNA clones provided the source of the probes. In total, 47 RFLP and 100 RAPD loci have been placed on the linkage map. A rather low DNA polymorphism rate (18% for RFLP markers and 29% for RAPD primers) was detected. Only 81% of RAPD markers and 85% of RFLP markers fit an expected 1:1 ratio The availability of a molecular linkage map has many implications for the future development of the genetics and breeding of this commercially important crop species.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesLashermes, P. ; Cros, J. ; Combes, M. C. ; Trouslot, P. ; Anthony, F. ; Hamon, S. ; Charrier, A.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Key words Chloroplast DNA ; Coffea ; Inheritance ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract CpDNA variation among 52 tree samples belonging to 25 different taxa of Coffea and two species of Psilanthus was assessed by RFLP analysis on both the total chloroplast genome and the atpB-rbcL intergenic region. Twelve variable characters were distinguished allowing the identification of 12 different plastomes. The low sequence divergence observed might suggest that Coffea is a young genus. The results were in contradiction with the present classification into two genera. Additionally, cpDNA inheritance was studied in interspecific hybrids between C. arabica and C. canephora, and in an intraspecific progeny of C. canephora, using PCR-based markers. Both studies showed exclusively maternal inheritance of cpDNA.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesLashermes, P. ; Combes, M.-C. ; Robert, J. ; Trouslot, P. ; D'Hont, A. ; Anthony, F. ; Charrier, A.
Springer
Published 1999Staff ViewISSN: 1617-4623Keywords: Key words Africa ; Amphidiploidy ; Coffee tree ; Molecular cytogenetics ; Restriction fragment length polymorphismSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Restriction fragment length polymorphism (RFLP) markers were used in combination with genomic in situ hybridisation (GISH) to investigate the origin of the allotetraploid species Coffea arabica (2n = 44). By comparing the RFLP patterns of potential diploid progenitor species with those of C. arabica, the sources of the two sets of chromosomes, or genomes, combined in C. arabica were identified. The genome organisation of C. arabica was confirmed by GISH using simultaneously labelled total genomic DNA from the two putative genome donor species as probes. These results clearly suggest that C. arabica is an amphidiploid formed by hybridisation between C. eugenioides and C. canephora, or ecotypes related to these diploid species. Our results also indicate low divergence between the two constituent genomes of C. arabica and those of its progenitor species, suggesting that the speciation of C. arabica took place relatively recently. Precise localisation in Central Africa of the site of the speciation of C. arabica, based on the present distribution of the coffee species, appears difficult, since the constitution and extent of tropical forest has varied considerably during the late Quaternary period.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1573-5060Keywords: Coffea arabica ; coffee ; diversity ; genetic resources ; heterosis ; RAPDSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Random amplified polymorphic DNA (RAPD) markers have been successfully employed to analyse the genetic diversity among cultivated and subspontaneous accessions of Coffea arabica. The narrow genetic base of commercial cultivars was confirmed. On the other hand, a relatively large genetic diversity was observed within the germplasm collection demonstrating the importance of collecting missions. Results suggested an East-West differentiation in Ethiopia, the primary centre of diversification of C. arabica. The large heterosis effect reported in intergroup hybrids could be related to such genetic differentiation. RAPD method appeared to be effective in resolving genetic variations and in grouping germplasm in C. arabica.Type of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 1573-5109Keywords: Coffea ; diversity ; PCR ; arbitrary primersSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary The use of random amplified DNA fragments as genetic markers in Coffea was investigated. Arbitrary oligonucleotides were used as primers to amplify genomic DNA of different coffee accessions representing major Coffea species by polymerase chain reaction. Intraspecific variation was easily detected in C. canephora and C. liberica whereas the primers assayed failed to reveal polymorphism between C. arabica accessions. Extensive interspecific variation was observed. Genetic relationships between Coffea species are deduced from the degrees of similarity in amplified product profiles. Random amplified DNA markers appeared to be of high value for characterization, analysis and utilization of coffee genetic resources.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 1573-5060Keywords: coffee ; doubled haploid ; fertility ; polyembryony ; inbreeding ; Coffea canephoraSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Doubled haploids (DH) of Coffea canephora Pierre were developed using haploid embryos which occur spontaneously in association with polyembryony. The frequencies of polyembryonic seeds and haploid embryos varied according to the parental genotypes. However, production of a large number of DH seemed possible from all genotypes. More than 750 DHs produced from various genotypes were grown under field conditions and evaluated for different characters of agronomic importance. Approximately half of DH genotypes did not survive, suggesting a strong, negative effect of homozygosity. Inbreeding depression is particularly severe on general vigor and reproductive aspects. For several characters studied such as leaf shape, leaf rust resistance and hundred bean weight, considerable genetic variations were observed within and between groups of DHs constituted by the DHs produced from the same clone. Despite their low vigor and reduced fertility, the DHs of C. canephora offer new possibilities in genetic research and coffee breeding.Type of Medium: Electronic ResourceURL: