Search Results - (Author, Cooperation:P. L. Davies)
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1Latest Papers from Table of Contents or Articles in PressT. Sun ; F. H. Lin ; R. L. Campbell ; J. S. Allingham ; P. L. Davies
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-02-18Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Alanine/chemistry ; Animals ; Antifreeze Proteins, Type I/*chemistry ; Crystallography, X-Ray ; Fish Proteins/*chemistry ; Flounder ; Ice ; *Protein Folding ; Protein Structure, Secondary ; Water/chemistryPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1095-8649Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: The production of transgenic salmon using gene transfer technology is described. Both antifreeze proteins and growth hormone genes have been successfully transferred. The expression, inheritance and phenotypes are examined using a wide variety of techniques. The development of new transgenics will be beneficial to aquaculture.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Fig. 1. Solubility of Water in Benzene and Toluene at 20C. A stream of dry air was saturated, with tritiatel water at temperature Tl and was allowed to come to equilibrium with the hydrocarbon liquid at temperature T2 (T2〉T'1). Samples were taken of the hydrocarbon after equilibration and ...Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1432-0614Source: Springer Online Journal Archives 1860-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract Sea raven type II antifreeze protein (SRAFP) is one of three different fish antifreeze proteins isolated to date. These proteins are known to bind to the surface of ice and inhibit its growth. To solve the three-dimensional structure of SRAFP, study its ice-binding mechanism, and as a basis for engineering these molecules, an efficient system for its biosynthetic production was developed. Several different expression systems have been tested including baculovirus, Escherichia coli and yeast. The latter, using the methylotrophic organism Pichia pastoris as the host, was the most productive. In shake-flask cultures the levels of SRAFP secreted from Pichia were up to 5 mg/l. The recombinant protein has an identical activity to SRAFP from sea raven serum. In order to increase yields further, four different strategies were tested in 10-l fermentation vessels, including: (1) optimization of pH and dissolved oxygen, (2) mixed feeding of methanol and glycerol with Muts clones, (3) supplementation of amino acid building blocks, and (4) methanol feeding with Mut+ clones. The mixed-feeding/Muts strategy proved to be the most efficient with SRAFP yields reaching 30 mg/l.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1617-4623Keywords: Key wordsDrosophila melanogaster ; P-elements ; Antifreeze protein ; Intron ; Gene regulationSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Using Drosophila as a host, we have examined the effect that the presence of an intron has on the accumulation of a processed transgene mRNA. To provide a model system that was free from major position effects, two fish antifreeze protein (AFP) transgenes were arranged in divergent transcriptional orientation from a central Drosophila yolk protein promoter/enhancer region and introduced into the flies by P-element transformation and/or mobilization. In this way the organization of both the structural genes and the promoter elements mimicked their natural arrangements. When one member of the fish AFP transgene pair had its single 180 bp intron deleted, there was a 2- to 11-fold (average 5-fold) decrease in its mRNA level compared to that generated from the control gene containing the fish AFP intervening sequence. When the deleted intron was replaced by a 70 bp intervening sequence originating from the yolk protein 1 gene, mRNA accumulation was restored to its original level. Even for the streamlined genome of Drosophila, where the intron number and size are generally reduced compared to mammals, the presence of an intervening sequence appears to facilitate mRNA accumulation.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1618-2650Source: Springer Online Journal Archives 1860-2000Topics: Chemistry and PharmacologyNotes: Summary The paper criticizes the use of standard outlier tests when evaluating interlaboratory data. It is shown that many such tests are not able to detect gross outliers (masking effect). An alternative method of evaluation using tests of estimates based on robust statistics is proposed.Type of Medium: Electronic ResourceURL: