Search Results - (Author, Cooperation:P. Gepts)

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  1. 1
    Staff View
    Publication Date:
    2013-07-05
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Acclimatization/genetics ; Agriculture/economics/*methods/*trends ; Biodiversity ; Biological Specimen Banks ; Breeding ; Crops, Agricultural/genetics ; Food Supply/*statistics & numerical data ; Genes, Plant ; Humans ; Phenotype ; Seeds/genetics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Schinkel, Christiane ; Gepts, P.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1989
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    The tepary bean (Phaseolus acutifolius) is the object of renewed interest because it possesses some interesting agronomic attributes such as tolerance to drought and salinity. In order to gain a better understanding of the organisation of its genetic diversity, we have examined patterns of diversity for nine polymorphic enzyme systems representing 12 loci, in a sample of 55 wild and 8 cultivated accessions. Several geographic patterns were identified, including an East-West differentiation, (across the Sierra Madre Occidental mountains), a North-South differentiation, and a localized differentiation. Wild teparies displayed a higher number of polymorphic loci, as well as a higher number of alleles per polymorphic locus compared to cultivars, consistent with previous observations indicating a reduction of phaseolin diversity on domestication. No clearcut separation with respect to isozyme differentiation was observed between P. acutifolius var. tenuifolius and var. acutifolius, further questioning the validity of this taxonomic separation based on leaflet shape.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1439-0523.1989.tb00336.x

    Articles: DFG German National Licenses
  3. 3
    Schinkel, Christiane ; Gepts, P.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1988
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    The tepary bean (Phaseolus acutifolius A. Gray) is grown mainly in and regions of Mexico and the southwestern U.S. as a subsistence crop by small farmers. It is also a store of genetic variability for traits such as disease and pest resistance and stress tolerance to improve the common bean (P. vulgaris L.). To determine geographic patterns of variation and the influence of domestication on genetic variability, the genetic diversity of phaseolin, the major seed storage protein, was characterized among 55 wild and 8 cultivated teparies using polyacrylamide gel electrophoresis. Fifteen electrophoretic phaseolin patterns were identified among wild forms, whereas only one pattern was exhibited by cultivars. This result suggests a single domestication in this species leading to a strong reduction in diversity. An additional finding is the divergence m phaseolin types between populations east and west of the Sierra Madre Occidental mountains.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1439-0523.1988.tb00301.x

    Articles: DFG German National Licenses
  4. 4
    Gepts, P.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0167-7799
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0167-7799(91)90133-3

    Articles: DFG German National Licenses
  5. 5
    Gepts, P. ; Bliss, F. A.
    Springer
    Published 1984
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Phaseolus vulgaris L. ; Streptococcus zymogenes bioassay ; Rocket immunoelectrophoresis ; Micro-Kjeldahl ; Limiting essential amino acid
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary The relationship between available methionine concentration and the levels of phaseolin — the major seed storage proteins of the common bean — was studied using three groups of genetic materials: First, the F2 progenies of interspecific crosses between P. vulgaris cultivars and aP. coccineus subsp. coccineus line (cv. ‘Mexican Red Runner’) having no detectable phaseolin; second, the F2 progenies and segregating F3 families of crosses between cultivated P. vulgaris lines and a Mexican wild bean accession (PI 325690-3) carrying a gene producing a reduction in phaseolin content; third, two inbred backcross populations: ‘Sanilac’x‘Bush Blue Lake 240’ (population 2) and ‘Sanilac’x‘15R 148’ (population 6). Total seed N levels were determined by micro-Kjeldahl, phaseolin levels by rocket immunoelectrophoresis and available methionine levels by the Streptococcus zymogenes bioassay. Our results indicate that in all the genetic materials studied, with the exception of population 6, higher phaseolin levels lead to increased available methionine concentration. Although phaseolin has a low methionine concentration, it is actually a major source of available methionine in common bean seeds, because it represents a large part of total seed nitrogen and because limited differences exist between the methionine concentrations of the different protein fractions. This contrasts with the situation in cereals such as maize, barley and sorghum, where increased levels of the major limiting amino acid (lysine) can be achieved through a decrease in the amounts of the main seed storage protein fraction (prolamines). In population 6, no relationship was observed between available methionine and phaseolin content. Other factors, such as additional methionine-rich polypeptides or the presence of tannins, might obscure the positive relationship between phaseolin and available methionine content in population 6.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00262537

    Articles: DFG German National Licenses
  6. 6
    Llaca, V. ; Delgado Salinas, A. ; Gepts, P.
    Springer
    Published 1994
    Staff View
    ISSN:
    1432-2242
    Keywords:
    cpDNA ; Phaseolus vulgaris ; Phaseolus coccineus ; Phylogeny ; Molecular evolution
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract We have analyzed the changes occurring in the chloroplast DNA (cpDNA) of taxa belonging to thePhaseolus vulgaris complex to help clarify relationships among species of this complex. Two restriction maps for 11 restriction enzymes comprising the whole chloroplast genome from a wildP. vulgaris and a wildP. coccineus accession were constructed. These maps allowed us to compare a total of 330 restriction sites between the two genomes in order to identify polymorphisms, assess the type of mutations detected, and identify regions of high variability. A region, located in the large single-copy region near the borders with the inverted repeats, accounted for a large portion of the variation. Most of the mutations detected were due to restriction sites gains or losses. Variable and conserved regions were then evaluated in 30 accessions belonging to taxa of theP. vulgaris complex. Phylogenetic analyses were made using parsimony methods. Conclusions obtained from such analyses were the following: (1) there was high cpDNA variability withinP. coccineus but not inP. vulgaris. (2)P. coccineus subsp.glabellus showed a very distinct cpDNA type that strongly suggests that it actually belongs to a different but as yet undetermined section of the genus. Our cpDNA observations are supported by distinctive morphological traits and reproductive biology of this taxon. (3) InP. coccineus subsp.darwinianus (also classified asP. polyanthus), the cpDNA lineage was in disagreement with data obtained from nuclear markers and suggested a reticulated origin by hybridization betweenP. coccineus as the male parent and an ancestralP. polyanthus type, closely allied toP. vulgaris, as the seed parent. This initial cross was presumably followed by repeated backcrossing toP. coccineus. Our cpDNA studies illustrate the importance of molecular markers in elucidating phylogenetic relationships. They also indicate that accurate phylogenies will require analyses of both nuclear and cytoplasmic genomes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF01253966

    Articles: DFG German National Licenses
  7. 7
    Menéndez, C. M. ; Hall, A. E. ; Gepts, P.
    Springer
    Published 1997
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Vigna unguiculata ; RFLP ; RAPD ; AFLP ; Linkage map
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  We have constructed a genetic linkage map within the cultivated gene pool of cowpea (2n=2x=22) from an F8 recombinant inbred population (94 individuals) derived from a cross between the inbreds IT84S-2049 and 524B. These breeding lines, developed in Nigeria and California, show contrasting reactions against several pests and diseases and differ in several morphological traits. Parental lines were screened with 332 random RAPD decamers, 74 RFLP probes (bean, cowpea and mung bean genomic DNA clones), and 17 AFLP primer combinations. RAPD primers were twice as efficient as AFLP primers and RFLP probes in detecting polymorphisms in this cross. The map consists of 181 loci, comprising 133 RAPDs, 19 RFLPs, 25 AFLPs, three morphological/classical markers, and a biochemical marker (dehydrin). These markers identified 12 linkage groups spanning 972 cM with an average distance of 6.4 cM between markers. Linkage groups ranged from 3 to 257 cM in length and included from 2 to 41 markers, respectively. A gene for earliness was mapped on linkage group 2. Seed weight showed a significant association with a RAPD marker on linkage group 5. This map should facilitate the identification of markers that “tag” genes for pest and disease resistance and other traits in the cultivated gene pool of cowpea.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050683

    Articles: DFG German National Licenses
  8. 8
    Osborn, T. C. ; Blake, T. ; Gepts, P. ; Bliss, F. A.
    Springer
    Published 1986
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Phaseolus vulgaris ; Seed protein ; Arcelin ; Inheritance ; Linkage ; Bruchidae
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary Crude proteins from seeds of wild bean accessions of Mexican origin were analyzed by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS/PAGE). Several accessions had electrophoretic patterns showing unique protein bands. When analyzed by two-dimensional isoelectric focusing (IEF)-SDS/PAGE, four protein variants which had electrophoretic mobilities similar to each other but different from the other major seed proteins, phaseolin and lectin, were observed. All four variants, which have not been described in cultivated beans, were tentatively named arcelin proteins and designated as arcelin 1, 2, 3 and 4. Arcelins 3 and 4 had polypeptides that comigrated on two-dimensional gels and these variants occurred in accessions that were collected in the same location. Analysis of single F2 seeds from crosses among arcelin-containing lines and from crosses between cultivated beans lines without arcelin and arcelin-containing lines revealed that differences in arcelin polypeptide expression were inherited monogenically. The alleles for different arcelin variants were codominant to each other and dominant to the absence of arcelin. The gene(s) controlling arcelin proteins were unlinked to those controlling phaseolin expression and tightly linked to genes controlling the presence of lectin proteins (〈 0.30% recombination). The possible origins of arcelin genes and their potential role in bruchid resistance are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00276428

    Articles: DFG German National Licenses
  9. 9
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Crop evolution ; M13 ; Fingerprinting ; Linkage map
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract M13 DNA fingerprinting was used to determine evolutionary changes that occurred in Latin American germ plasm and USA cultivars of commonbean (Phaseolus vulgaris L.) during domestication. Linkage mapping experiments showed that M13-related sequences in the common-bean genome were either located at the distal ends of linkage groups or that they were unlinked to each other or to any previously mapped markers. Levels of polymorphism observed by hybridization with M13 (1 probe-enzyme combination) were comparable to those observed by hybridization with single-copy random PstI genomic probes (36 enzyme-probe combinations) but were higher than those observed for isozymes (10 loci). Results indicated that the wild ancestor had diverged into two taxa, one distributed in Middle America (Mexico, Central America, and Colombia) and the other in the Andes (Peru and Argentina); they also suggested separate domestications in the two areas leading to two cultivated gene pools. Domestication in both areas led to pronounced reductions in diversity in cultivated descendants in Middle America and the Andes. The marked lack of polymorphism within commercial classes of USA cultivars suggests that the dispersal of cultivars from the centers of origin and subsequent breeding of improved cultivars led to high levels of genetic uniformity. To our knowledge, this is the first crop for which this reduction in diversity has been documented with a single type of marker in lineages that span the evolution between wild ancestor and advanced cultivars.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00222458

    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Phaseolus vulgaris L. ; Core linkage map ; RFLPs ; RAPDs
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  Three RFLP maps, as well as several RAPD maps have been developed in common bean (Phaseolus vulgaris L.). In order to align these maps, a core linkage map was established in the recombinant inbred population BAT93×Jalo EEP558 (BJ). This map has a total length of 1226 cM and comprises 563 markers, including some 120 RFLP and 430 RAPD markers, in addition to a few isozyme and phenotypic marker loci. Among the RFLPs mapped were markers from the University of California, Davis (established in the F2 of the BJ cross), University of Paris-Orsay, and University of Florida maps. These shared markers allowed us to establish a correspondence between the linkage groups of these three RFLP linkage maps. In total, the general map location (i.e., the linkage group membership and approximate location within linkage groups) has been determined for some 1070 markers. Approaches to align this core map with other current or future maps are discussed.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050964

    Articles: DFG German National Licenses
  11. 11
    Koenig, R. ; Gepts, P.
    Springer
    Published 1989
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Crop evolution ; Domestication ; Molecular markers ; Common bean
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary Allozyme analysis was performed on 83 wild Phaseolus vulgaris accessions, representing a wide geographical distribution from Mesoamerica to Argentina, to determine levels of genetic diversity and geographic patterns of variability at nine polymorphic isozyme loci. The collection can be divided into two major groups, one consisting of accessions from Mexico, Central America, Colombia and Peru, and the other consisting of accessions from Peru and Argentina. One accession from northern Peru is distinct from the two major groups, and may delineate a transition zone between the two divergent groups. The level of genetic diversity within wild P. vulgaris (Ht=0.132) is comparable with those found in other Phaseolus species. There was no significant within-accession gene diversity (Hs=0.006); however, there is a moderate level of genetic diversity (Dst=0.126) between accessions. Our results are consistent with previous studies on the genetic diversity of wild P. vulgaris using phaseolin, the major seed storage protein of beans.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00266663

    Articles: DFG German National Licenses
  12. 12
    Nodari, R. O. ; Koinange, E. M. K. ; Kelly, J. D. ; Gepts, P.
    Springer
    Published 1992
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Phaseolus vulgaris ; RFLP ; Genetic diversity
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary Two genomic libraries were established to provide markers to develop an integrated map combining molecular markers and genes for qualitative and quantitative morpho-agronomic traits in common bean. Contrasting characteristics were observed for the two libraries. While 89% of the PstI clones were classified as single-copy sequences, only 21% of the EcoRIBamHI clones belonged in that category. Clones of these two libraries were hybridized against genomic DNA of nine genotypes chosen according to their divergent evolutionary origin and contrasting agronomic traits. Eight restriction enzymes were used in this study. PstI clones revealed 80–90% polymorphism between the Andean and Middle American gene pools and 50–60% polymorphism within these gene pools. However, under the same conditions only 30% of the EcoRI-BamHI clones showed polymorphism between the Middle American and Andean gene pools. Hybridization with PstI clones to EcoRI-, EcoRV-, or HindIII-digested genomic DNA resulted in a cumulative frequency of polymorphism of approximately 80%. Hybridizations to BamHI-, HaeIII-, HinfI-, PstI-, and XbaI-digested genomic DNA detected no additional polymorphisms not revealed by the former three enzymes. In the PstI library, a positive correlation was observed between the average size of hybridizing restriction fragments and the frequency of polymorphism detected by each restriction enzyme. This relationship is consistent with the higher proportion of insertion/deletion events compared with the frequency of nucleotide substitutions observed in that library.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00223999

    Articles: DFG German National Licenses
  13. 13
    Nodari, R. O. ; Tsail, S. M. ; Gilbertson, R. L. ; Gepts, P.
    Springer
    Published 1993
    Staff View
    ISSN:
    1432-2242
    Keywords:
    RFLP ; RAPD ; Linkage map ; Bean Common Mosaic Virus resistance ; Segregation distortion
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary A restriction fragment length polymorphism (RFLP)-based linkage map for common bean (Phaseolus vulgaris L.) covering 827 centiMorgans (cM) was developed based on a F2 mapping population derived from a cross between BAT93 and Jalo EEP558. The parental genotypes were chosen because they exhibited differences in evolutionary origin, allozymes, phaseolin type, and for several agronomic traits. The segregation of 152 markers was analyzed, including 115 RFLP loci, 7 isozyme loci, 8 random amplified polymorphic DNA (RAPD) marker loci, and 19 loci corresponding to 15 clones of known genes, 1 virus resistance gene, 1 flower color gene, and 1 seed color pattern gene. Using MAPMAKER and LINKAGE-1, we were able to assign 143 markers to 15 linkage groups, whereas 9 markers remained unassigned. The average interval between markers was 6.5 cM; only one interval was larger than 30 cM. A small fraction (9%) of the markers deviated significantly from the expected Mendelian ratios (1∶2∶1 or 3∶1) and mapped into four clusters. Probes of known genes belonged to three categories: seed proteins, pathogen response genes, and Rhizobium response genes. Within each category, sequences homologous to the various probes were unlinked. The I gene for bean common mosaic virus resistance is the first disease resistance gene to be located on the common bean genetic linkage map.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00220907

    Articles: DFG German National Licenses
  14. 14
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Disease resistance ; Anthracnose (Colletotrichum lindemuthianum) ; Common bean (Phaseolus vulgaris) ; Marker-assisted selection ; Leucine-rich repeat motif
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  Molecular markers offer new opportunities for breeding for disease resistance. Resistance gene pyramiding in a single cultivar, as a strategy for durable resistance, can be facilitated by marker-assisted selection (MAS). A RAPD marker, ROH20450, linked to the Mesoamerican Co-2 anthracnose resistance gene, was previously transformed into a SCAR marker, SCH20. In the present paper we have further characterized the relevance of the SCH20 SCAR marker in different genetic backgrounds. Since this SCAR marker was found to be useful mainly in the Andean gene pool, we identified a new PCR-based marker (SCAreoli) for indirect scoring of the presence of the Co-2 gene. The SCAreoli SCAR marker is polymorphic in the Mesoamerican as well as in the Andean gene pool and should be useful in MAS. We also report that PvH20, the cloned sequence corresponding to the 450-bp RAPD marker ROH20450, contains six imperfect leucine-rich repeats, and reveals a family of related sequences in the vicinity of the Co-2 locus. These results are discussed in the context of the recent cloning of some plant resistance genes.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220050766

    Articles: DFG German National Licenses
  15. 15
    Staff View
    ISSN:
    1573-5036
    Keywords:
    chalcone isomerase ; chalcone synthetase ; chitinase ; PAL ; phaseolin ; Phaseolus vulgaris ; QTL ; RFLP ; Rhizobium tropici ; Xanthomonas campestris bv. phaseoli
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Abstract A recently developed bean RFLP linkage map was used to identify genetic elements affecting quantitative trait loci (QTLs) in two contrasting common bean genotypes, BAT-93 and Jalo EEP558, under two levels of mineral nitrogen: low – 0.25 mM NH4NO3 and a high – 6 mM NH4NO3. QTLs affecting nodule number (NN) and response to Xanthomonas campestris bv. phaseoli, which causes common bacterial blight (CBB) were identified and mapped. Analyses of 70 F2-derived F3 families, using the F1, the two parents, and a nodulation-defective mutant (Nod-) inoculated with R. tropici UM1899 under both levels of N showed significant differences (P#60;0.0001) among the F3 families for NN. Under low N, three genomic regions influenced both traits, with seven linked markers. In three of the six regions influencing NN, higher NN was associated with the Jalo EEP-558 allele, whereas in only two regions was the BAT-93 allele associated with higher NN. One-way analysis of variance, with each marker as the independent variable and NN as the dependent variable, and interval mapping analysis identified four QTLs, which accounted for 45% of the total variation, and two additional QTLs near to yet unassigned loci. In linkage group D7, one QTL mapped to the same region as a QTL for CBB. Under high N, three additional regions were linked to NN, one where the BAT-93 allele was closely associated with CH18 (chitinase), and the others where the Jalo EEP-558 allele was associated with CHS (chalcone synthetase) and PAL-1 (phenylalanine ammonia lyase). Four regions for CBB were mapped adjacent to or in the same region as a QTL for NN. Thus, N showed dual and opposite effects on the expression of NN and CBB. Analysis of these RFLP markers revealed these ‘hidden’ favorable alleles and can serve as an indirect selection tool to increase NN and resistance to CBB.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1023/A:1004347515127

    Articles: DFG German National Licenses
  16. 16
    Gutiérrez Salgado, A. ; Gepts, P. ; Debouck, D. G.
    Springer
    Published 1995
    Staff View
    ISSN:
    1573-5109
    Keywords:
    seed storage protein ; electrophoresis ; domestication ; crop evolution
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Summary The lima bean,Phaseolus lunatus L., is a bean species with a broad distribution in the Americas that rivals that of common bean (P. vulgaris). In order to better understand the organization of genetic diversity and the pattern of domestication in lima bean, a review was conducted of the available information on the geographic distribution of wild and cultivated forms of this species. In addition, one-dimensional SDS polyacrylamide gel electrophoresis of seed proteins was also conducted on a sample of 84 wild, 6 weedy, and 426 cultivated forms. Results show that wild forms can be divided into two groups, one with smaller seeds and a very extensive distribution that includes Mexico, Central America, and the eastern slope of the Andes, and the other with a more circumscribed distribution on the western slope of the Andes in Ecuador and northern Peru. Electrophoretic analyses of seed proteins confirmed this subdivision and, additionally, showed that the large-seeded cultivars had been domesticated from the large-seeded wild lima beans in western South America. For the small-seeded lima bean cultivars, it was not possible to determine a domestication center as the most abundant protein pattern in the cultivars also had a widespread distribution in the small-seeded wild progenitor. Electrophoretic analyses showed, however, that domestication led to a reduction of genetic diversity in the small-seeded, Mesoamerican group, but not in the large-seeded group. The latter may be due to insufficient sampling of the larger-seeded, wild germplasm.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF02310680

    Articles: DFG German National Licenses
  17. 17
    Stockton, T. ; Gepts, P.
    Springer
    Published 1994
    Staff View
    ISSN:
    1573-5060
    Keywords:
    M13 ; minisatellite ; common bean ; Phaseolus vulgaris ; genetic diversity ; RFLP ; DNA fingerprinting
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Summary Analyses of genetic diversity within populations could be of great benefit to plant genetic resources conservation. In order to identify genetic markers that are variable within populations, the genome of Phaseolus vulgaris was screened with several DNA sequences in order to identify hypervariable sequences. Polymorphisms were observed between Middle American and Andean cultivars using the protein III tandem repeat of the M13 phase and the 33.15 human minisatellite. Extensive differences were observed when the DNA of two divergent lines—BAT93 and Jalo EEP558, of Middle American and Andean origin, respectively—were digested with HinfI, TaqI, HaeIII and hybridized with the 33.15 human minisatellite. Similarly, numerous polymorphisms were observed when the M13 protein III tandem repeat region was hybridized with TaqI digests of these cultivars. Polymorphism was also detected among sister lines of two F6 backcross materials involving Middle American and Andean lines when genomic DNA was digested with TaqI and hybridized with M13 tandem repeat region. In addition, polymorphism was observed among Porrillo cultivars that resulted from selection within a single landrace population. Whereas only one isozyme difference had been observed previously among the Porrillo cultivars, eleven restriction fragments detected by the M13 protein III tandem repeat sequence differentiated these cultivars. Ribosomal DNA also hybridized to several polymorphic bands on TaqI and EcoRI genomic Southern blots of the F6 backcross material. Only one polymorphism was observed with EcoRI-digested genomic DNA of BAT93 and Jalo EEP558 was hybridized with microsatellite (GACA)4. This probe might be useful in ascertaining relationships at the species and subspecies level, and as a marker in mapping studies. Our results show that both the human 33.15 minisatellite and M13 should be useful probes to detect within-population variability in common bean.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00022162

    Articles: DFG German National Licenses