Search Results - (Author, Cooperation:L. Mu)
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1Latest Papers from Table of Contents or Articles in PressLyu, N., Lin, Y., Kong, Y., Zhang, Z., Liu, L., Zheng, L., Mu, L., Wang, J., Li, X., Pan, T., Xie, Q., Liu, Y., Lin, A., Wu, P., Zhao, M.
BMJ Publishing Group
Published 2018Staff ViewPublication Date: 2018-01-10Publisher: BMJ Publishing GroupPrint ISSN: 0017-5749Electronic ISSN: 1468-3288Topics: MedicineKeywords: GutPublished by: -
2Latest Papers from Table of Contents or Articles in PressBasen, M., Geiger, I., Henke, L., Müller, V.
The American Society for Microbiology (ASM)
Published 2018Staff ViewPublication Date: 2018-01-18Publisher: The American Society for Microbiology (ASM)Print ISSN: 0099-2240Electronic ISSN: 1098-5336Topics: BiologyPublished by: -
3Latest Papers from Table of Contents or Articles in PressDeng, L., Mu, R., Weston, T. A., Spencer, B. L., Liles, R. P., Doran, K. S.
The American Society for Microbiology (ASM)
Published 2018Staff ViewPublication Date: 2018-06-22Publisher: The American Society for Microbiology (ASM)Print ISSN: 0019-9567Electronic ISSN: 1098-5522Topics: MedicinePublished by: -
4Latest Papers from Table of Contents or Articles in PressStaff View
Publication Date: 2018-02-03Publisher: Institute of Physics (IOP)Print ISSN: 1757-8981Electronic ISSN: 1757-899XTopics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsPublished by: -
5Latest Papers from Table of Contents or Articles in PressS. Z. Shen ; J. L. Crowley ; Y. Wang ; S. A. Bowring ; D. H. Erwin ; P. M. Sadler ; C. Q. Cao ; D. H. Rothman ; C. M. Henderson ; J. Ramezani ; H. Zhang ; Y. Shen ; X. D. Wang ; W. Wang ; L. Mu ; W. Z. Li ; Y. G. Tang ; X. L. Liu ; L. J. Liu ; Y. Zeng ; Y. F. Jiang ; Y. G. Jin
American Association for the Advancement of Science (AAAS)
Published 2011Staff ViewPublication Date: 2011-11-19Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Biodiversity ; Carbon Dioxide ; Carbon Isotopes ; China ; *Ecosystem ; *Extinction, Biological ; Fires ; *Fossils ; Geologic Sediments ; Invertebrates/classification ; Isotopes ; Lead ; Mass Spectrometry ; Methane ; Oceans and Seas ; Plants/classification ; Radioisotope Dilution Technique ; Radiometric Dating ; Seawater/chemistry ; Time ; Uranium ; Vertebrates/classificationPublished by: -
6Latest Papers from Table of Contents or Articles in PressKramer, S. D., Betzel, T., Mu, L., Haider, A., Herde, A. M., Boninsegni, A. K., Keller, C., Szermerski, M., Schibli, R., Wunsch, B., Ametamey, S. M.
The Society of Nuclear Medicine (SNM)
Published 2018Staff ViewPublication Date: 2018-04-03Publisher: The Society of Nuclear Medicine (SNM)Print ISSN: 0022-3123Topics: MedicinePublished by: -
7Latest Papers from Table of Contents or Articles in PressY. Xu, W. Cai, Y. Ma, X. Mu, L. Hu, Tao Chen, H. Wang, Y. P. Song, Zheng-Yuan Xue, Zhang-qi Yin, and L. Sun
American Physical Society (APS)
Published 2018Staff ViewPublication Date: 2018-09-12Publisher: American Physical Society (APS)Print ISSN: 0031-9007Electronic ISSN: 1079-7114Topics: PhysicsKeywords: General Physics: Statistical and Quantum Mechanics, Quantum Information, etc.Published by: -
8Articles: DFG German National LicensesMu, L. J. ; Gaudernack, G. ; Sæbøe-Larssen, S. ; Hammerstad, H. ; Tierens, A. ; Kvalheim, G.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: With the aim of producing large quantities of mRNA-transfected monocyte-derived dendritic cells (DCs) to be used as cancer vaccines, a new clinical grade procedure has been developed. Peripheral blood mononuclear cells (PBMCs) obtained by leukapheresis were enriched for monocytes by immunomagnetic depletion of CD19+ B cells and CD2+ T cells employing the ISOLEX 300i device. After 5 days of culture of enriched monocytes in gas permeable Teflon bags, using serum-free medium supplemented with granulocyte/macrophage-colony stimulating factor and interleukin-4 (IL-4), immature DCs were generated. Following transfection with mRNA from three human prostate cancer cell lines (DU145, LNCaP and PC-3), employing a newly developed square wave electroporation procedure, the immature DCs were immediately transferred to Teflon bags and matured for 48 h, using serum-free medium supplemented with IL-1α, IL-6, tumour necrosis factor-α and PGE2. The electroporation procedure efficiently transferred mRNA into the DCs with minor effect on the viability of the cells. The generated matured transfected DCs show high expression of the antigens CD83, CD80, CD86 and human leucocyte antigen-DR. Freezing and thawing of the transfected matured DCs had minor effect on cell viability and the phenotype. From 4 × 109 PBMCs, about 1 × 108 transfected matured DCs are produced. The thawed transfected DCs were able to elicit primary T-cell responses in vitro against antigens encoded by the prostate cancer mRNA as shown by enzyme-linked immunospot assay using mock-transfected DCs as control. Based on these results, clinical trials in cancer patients have been initiated.Type of Medium: Electronic ResourceURL: