Search Results - (Author, Cooperation:L. Morelli)

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  1. 1
  2. 2
    Staff View
    Publication Date:
    2013-08-03
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Adult ; Chromosomes, Human, Y/*classification/*genetics ; European Continental Ancestry Group/*genetics ; *Evolution, Molecular ; Haplotypes ; Humans ; Italy ; Male ; Phylogeny ; Polymorphism, Single Nucleotide
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  3. 3
    SQUIQUERA, L. ; GALIMBERTI, R. ; MORELLI, L. ; PLOTKIN, L ; MILICICH, R ; KOWALCKZUK, A. ; LEONI, J

    Oxford, UK : Blackwell Publishing Ltd
    Published 1994
    Staff View
    ISSN:
    1365-2230
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    In order to analyse the humoral immune response to the commensal yeast Pityrosporum ovale, we developed a western immunoblot technique with a salt soluble extract of P. ovale cytoplasm. In the present study, we tested sera from patients with psoriasis (n= 15), seborrhoeic dermatitis (n= 10), pityriasis versicolor (n= 8), and normal controls (n = 10). Seventy-three per cent (11/15) of the patients with psoriasis showed specific reactivity with a protein derived from P. ovale of estimated molecular mass 120 kDa, and 46% (7/15) of the cases recognized a 100-kDa protein. Sera from pityriasis versicolor and normal donors showed nonspecific reactivity with several bands of lower molecular weight.To characterize the location of the 100 and 120-kDa proteins, we performed a lyticase digestion of the cell wall, and analysed the soluble digested products by western blotting. The sera from psoriasis patients detected several bands in the range 100–120 kDa. The finding of the immunoreactive 120-kDa protein in this fraction suggests its location at the space between cell wail and membrane (periplasmic space).As a control, we performed an extraction of the cytoplasmic proteins of the dimorphic yeast Candida albicans. C. albicans showed a different pattern of banding in SDS–PAGE. Immunoblots with C. albicans did not allow the detection of any related band. A smear was observed in the high molecular weight range consistent with the presence of lipopolysaccharides.The role of the immune response in infection by P. ovale has not yet been fully explored. The function of the antibodies recognizing 100-120-kDa bands in the majority of the patients with psoriasis seems to represent a specific immune response to the yeast phase of P. ovale.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
    Staff View
    ISSN:
    0147-619X
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    0147-619X
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  6. 6
    Mokashi, A. ; Di Guilio, C. ; Morelli, L. ; Lahiri, S.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0034-5687
    Keywords:
    Carotid body, chronic hypoxia, loss of chemosensitivity ; Chemoreceptors ; Control of breathing ; Hypoxia, chronic ; Mammals, cat ; Transmitter, carotid body
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  7. 7
    Girlando, A. ; Morelli, L. ; Pecile, C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0009-2614
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  8. 8
    Girlando, A. ; Morelli, L. ; Pecile, C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0009-2614
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  9. 9
    Girlando, A. ; Morelli, L. ; Pecile, C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0009-2614
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  10. 10
    Gregory, W.D. ; Janik, R. ; Morelli, L.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0375-9601
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  11. 11
    Ventura, M. ; Callegari, M.L. ; Morelli, L.

    Oxford, UK : Blackwell Publishing Ltd
    Published 2000
    Staff View
    ISSN:
    1574-6968
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    The paper describes two methods that allow rapid and accurate identification of Lactobacillus helveticus strains based on the nucleotide sequence of the gene coding for the surface layer (S-layer) protein. The first method is based on a polymerase chain reaction amplification using primers targeting a specific fragment of the S-layer gene. The second method involved a portion of the S-layer gene of L. helveticus as a probe in a hybridisation test. The specificity of these protocols was checked for DNA samples isolated from various Lactobacillus strains.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  12. 12
    Mannu, L ; Paba, A ; Pes, M ; Floris, R ; Scintu, M.F ; Morelli, L

    Oxford, UK : Blackwell Publishing Ltd
    Published 1999
    Staff View
    ISSN:
    1574-6968
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Three molecular techniques (RAPD-polymerase chain reaction analysis, plasmid profile and pulsed-field gel electrophoresis) were used for a preliminary approach to type, at strain level, enterococci isolated from a 24-h-old home-made Pecorino Sardo (protected designation of origin) cheese. A high genetic polymorphism was found. Clusters obtained by the RAPD technique and plasmid profile analysis contained different strains. Pulsed-field gel electrophoresis proved to be an efficient and highly reproducible typing method. In addition, by combining the results from plasmid profile analysis and pulsed-field gel electrophoresis, it was possible to identify closely related strains probably belonging to the same clonal lineage.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  13. 13
    Cocconcelli, P.S. ; Morelli, L. ; Vescovo, M. ; Bottazzi, V.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1986
    Staff View
    ISSN:
    1574-6968
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Abstract A method to obtain gene transfer in lactic acid bacteria is described.When Streptococcus lactis SH4174 and Lactobacillus reuteri DSM20016 protoplasts undergo fusion by exposure to polyethylene glycol, plasmid pAMβ1 and trehalose-fermenting ability are transferred into L. reuteri.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  14. 14
    Vescovo, M. ; Morelli, L. ; Cocconcelli, P.S. ; Bottazzi, V.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1984
    Staff View
    ISSN:
    1574-6968
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Abstract A method for protoplast formation and regeneration suitable for Lactobacillus reuteri strains was developed.Lysozyme-treated cells formed protoplasts at a high percentage and regenerative ability varied according to the strains considered.Moreover, production and regeneration of protoplasts promoted the loss of plasmids harboured by the strains.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  15. 15
    Ferrero, M. ; Cesena, C. ; Morelli, L. ; Scolari, G. ; Vescovo, M.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1996
    Staff View
    ISSN:
    1574-6968
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Abstract Three different molecular techniques were used to characterize bacterial strains isolated from dairy products and phenotypically classified as Lactobacillus casei. Some strains were taxonomically identified as Lb. casei and Lb. paracasei using 23S rRNA-targeted probes specific for Lb. casei and Lb. paracasei. Chromosomal DNA was analyzed by pulsed-field gel electrophoresis. Lb. paracasei strains had a variable number of SfiI fragments harboring rRNA genes, while Lb. casei strains showed more homogeneous patterns. The chromosome size of Lb. paracasei ATCC 334 was evaluated to be 2.17 Mb.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  16. 16
    Morelli, L. ; Cesena, C. ; de Haën, C. ; Gozzini, L.
    Springer
    Published 1998
    Staff View
    ISSN:
    1432-184X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract Intestinal microbiota comprise a complex ecosystem whose equilibrium is crucial for the health of animal species. For humans, data exist on the microbiota composition in adult subjects, but few studies have addressed the microbiota composition in infants. In particular, data on the presence and species distribution of members of the genus Lactobacillus in newborns (less than one week old) are lacking. In the present work, the feces of healthy newborns were sampled to determine the taxonomic composition of Lactobacillus in the intestinal microbiota in a group of 16 neonates. In total, 1640 colony-forming units (CFU) were isolated, of which 420 grouped in the Lactobacillus genus by means of primary phenotypic characterization. The 420 isolates were further grouped into 125 strains on the basis of identical plasmid profiles. Of these 125 strains, 21 turned out to be permanent, i.e., they were identified in the feces of the same subject on several consecutive days. Sugar fermentation, DNA/DNA hybridization, and S-layer protein determination enabled us to classify 52 of the 125 strains as follows: L. paracasei (40 strains), L. delbrueckii sp. (1 strain), and L. acidophilus (sensu stricto) (11 strains). Based on the same criteria, the remaining 73 strains were tentatively allotted to the Johnson subgroup B, although hybridization experiments with probes specific for L. gasseri and L. johnsonii species were not performed. The presence of new species among these 73 strains cannot be excluded. Surprisingly, the obligately heterofermentative lactobacilli, L. reuteri in particular, were entirely absent from the feces of healthy newborns.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  17. 17
    Staff View
    ISSN:
    1573-0778
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Medicine
    Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  18. 18
    Staff View
    ISSN:
    1573-6784
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Summary We describe a rapid and reliable one-step method for purification of Lactobacillus secreted proteins. With electroendosmotic preparative electrophoresis and a modified synthetic medium the L. plantarum aggregation promoting factor, a 32 kDa secreted protein, and the thermostable α-amylase of Bacillus stearothermophilus cloned in L. reuteri were purified. Although the growth rate was reduced, the production of secreted proteins was not affected.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  19. 19
    Morelli, L. G. ; Cerdeira, H. A. ; Zanette, D. H.
    Springer
    Published 2005
    Staff View
    ISSN:
    1434-6036
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Physics
    Notes:
    Abstract. We analyze the phenomenon of frequency clustering in a system of coupled phase oscillators. The oscillators, which in the absence of coupling have uniformly distributed natural frequencies, are coupled through a small-world network, built according to the Watts-Strogatz model. We study the time evolution and determine variations in the transient times depending on the disorder of the network and on the coupling strength. We investigate the effects of fluctuations in the average frequencies, and discuss the definition of the threshold for synchronization. We characterize the structure of clusters and the distribution of cluster sizes in the synchronization transition, and define suitable order parameters to describe the aggregation of the oscillators as the network disorder and the coupling strength change. The non-monotonic behavior observed in some order parameters is related to fluctuations in the mean frequencies.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  20. 20
    Morelli, L. G. ; Abramson, G. ; Kuperman, M. N.
    Springer
    Published 2004
    Staff View
    ISSN:
    1434-6036
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Physics
    Notes:
    Abstract. We study a model of associative memory based on a neural network with small-world structure. The efficacy of the network to retrieve one of the stored patterns exhibits a phase transition at a finite value of the disorder. The more ordered networks are unable to recover the patterns, and are always attracted to non-symmetric mixture states. Besides, for a range of the number of stored patterns, the efficacy has a maximum at an intermediate value of the disorder. We also give a statistical characterization of the spurious attractors for all values of the disorder of the network.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses