Search Results - (Author, Cooperation:K. Hussain)

2018-01-30

Institute of Physics (IOP)

1757-8981

1757-899X

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

2011-10-08

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Amino Acid Substitution ; Cell Membrane/metabolism ; Cell Nucleus/metabolism ; Child ; Female ; Growth ; HeLa Cells ; Heterozygote ; Humans ; Hypoglycemia/*genetics/*metabolism ; Insulin/blood/metabolism ; Male ; Mosaicism ; *Mutation ; Pedigree ; Protein Interaction Domains and Motifs ; Proto-Oncogene Proteins c-akt/chemistry/*genetics/metabolism ; Signal Transduction

1572-901X

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Abstract New picoline adducts with carbamic acid [(furan-2-yl)methylene]hydrazide–CuII (CFMH) (1); thiocarbamic acid [(furan-2-yl)methylene]hydrazide–CuII (TFMH) (2); carbamic acid [(furan-2-yl)ethylidene]hydrazide–CuII (CFEH) (3), thiocarbamic acid [(furan-2-yl)ethylidene]hydrazide–CuII (TFEH) (4); carbamic acid [(thiophene-2-yl) methylene]hydrazide–CuII (CTMH) (5), thiocarbamic acid [(thiophene-2-yl)methylene]hydrazide–CuII (TTMH) (6), carbamic acid [(thiophene-2-yl)ethylidene]hydrazide–CuII (CTEH) (7), thiocarbamic acid [(thiophene-2-yl)ethylidene]hydrazide–CuII (TTEH) (8) have been prepared and characterized by analytical, i.r., electronic, e.s.r. and c.v. spectral data. The electronic spectra suggest distorted octahedral geometry for all the picoline adducts. E.s.r. g ∥ values lie between 2.251–2.286 at l.n.t. All the adducts undergo a quasi-reversible one-electron reduction in the range +0.47 to +0.51 V versus s.c.e., attributable to the CuIII/CuII redox couple. The electron transfer is much faster in the semicarbazone complexes than in the thiosemicarbazone complexes. All adducts showed increased nuclease activity in the presence of oxidant; the nuclease activity is compared with that of the parent copper(II) complexes.

Electronic Resource

1471-0528

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Objective To evaluate the effect of changed cervical screening policies on a steady population with low migratory tendencies.Design A retrospective analysis study.Setting Dundee and Angus, Scotland.Subjects All women who developed cervical carcinoma between 1957 and 1992.Main outcome measures The incidence of and mortality from cervical cancer after the introduction of organised cervical screening in 1962, according to age, stage, histology and screening history.Results The initial fall in incidence of cervical cancer seen in women between 35 and 54 years after the introduction of cervical screening was not sustained during the last 10 years of our study and appears to have been transferred to women aged 55 years and older instead. After 1976 an increase in the incidence of cervical cancer was seen in women under 35 years. The reduction in mortality from cervical cancer appears to have reached a plateau since 1976. No effect of cervical screening was seen on the incidence of adenocarcinoma of the cervix.Conclusions The effect of changed cervical screening policies has been shown for a small population for a period of 35 years. The incidence of the higher stages of squamous cervical cancer continues to fall. The increase in incidence of cervical cancer in women under 35 years confirms similar trends seen in other countries. A background mortality rate refractory to further intensification of screening appears to have been reached. Adenocarcinoma of the cervix appears to gain in importance as cervical screening policies are shown to have their effect on its squamous counterpart.

Electronic Resource

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

A new gene, parD, has been located at 88.5 min on the genetic map of E. coli. Cells carrying an amber mutation in this gene, together with a temperature-sensitive suppressor tRNA, are able to grow, synthesize DNA and divide at both 30°C and 42°C. At 42°C, however, they are defective both in the separation of replicated chromosomes and in the placement of septa. Both the amount of DNA and the number of septa per cell mass are normal in cells growing at 42°C: only the localization of the chromosomes and septa are altered. As a result, cells of random sizes are produced at 42°C and the smallest of these contain no DNA.

Electronic Resource

1365-2958

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Medicine

The phenotype of a recently-described mutant (OVG), conditionally defective in chromosome partitioning and septal positioning, was originally thought to be due to a new gene (parD) mapping at 88.4 min. We have now shown that, in addition to the parD mutation, OV6 carries a gyrAam mutation and that this mutation is probably responsible for the gross phenotype of the mutant. We have cloned the gyrA gene, identified the GyrA protein, sequenced the gyrA gene and flanking genes, cloned and sequenced the gyrAam, mutation, and identified its truncated product, in addition, we have identified the transcriptional start point of the gyrA gene. The E. coli GyrA protein has extensive homologies with Gyrase proteins of other organisms and weak sequence homologies with some eukaryotic cytoskeletal proteins.

Electronic Resource

0008-6215

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

1365-2559

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Basal cell adenocarcinoma is a rare and relatively recently characterized malignant salivary gland tumour, the malignant counterpart of basal cell adenoma. Diagnosis depends on finding features similar to adenoma but with an infiltrative growth pattern and exclusion of adenoid cystic carcinoma, sialoblastoma and basaloid squamous carcinoma. Basal cell adenocarcinoma is very rarely reported in minor salivary glands. We report three cases of basal cell adenocarcinoma affecting the labial, buccal and palatal minor salivary glands. One recurred following complete removal but with lesional disruption and further local wide excision appeared curative. A further lesion failed to recur in 5 years' follow-up despite marginal excision and a third after 3 years' follow-up. Basal cell adenocarcinoma is considered a low-grade malignancy, and in the minor glands wide excision and radiotherapy are recommended. However, the reported lesions appear to have a more indolent behaviour than previously reported lesions in minor glands.

Electronic Resource

0022-3697

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Physics

Electronic Resource

0014-5793

Fusion protein ; Protein A ; Proteolytically-cleavable sequence ; Ricin A chain

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0014-5793

(E. coli) ; Expression ; Immunotoxin ; Lectin ; Ricin B chain

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0040-4039

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0040-6090

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Three ponies were inoculated with plasma containing 104.8 TCID50 of equine infectious anemia virus (EIAV) and observed for 165 to 440 days. Each pony developed a febrile response within 3 weeks of infection during which a plasma viremia ⩾103.5 TCID50/ml was observed. Analyses of four isolates from sequential febrile episodes in a single pony were conducted by two-dimensional tryptic peptide maps and with monoclonal antibodies in immunoblots. Structural and antigenic alterations were observed in the envelope glycoproteins gp90 and gp45, with greatest variation in gp90. Specific IgG to EIAV gp90, gp45, and p26 of homologous and heterologous isolates was detectable by immunoblots within one month after infection although IgG levels to gp45 at this time were relatively low. The group-specific determinants of gp90 and gp45 were more antigenic than those of p26; however, binding of IgG to these determinants did not correlate with neutralization of EIAV as assayed in fetal equine kidney cells. Neutralizing antibodies were first detectable within two months of infection and only neutralized viruses isolated prior to serum collection. Neutralizing activity of sera collected later in the infection was broadly reactive regardless of the number of clinical episodes the donor had suffered.

Electronic Resource

0392-6737

Nonlocalized single-particle electronic states

Springer Online Journal Archives 1860-2000

Physics

Riassunto Le anomale riduzioni del gap di energia nei semiconduttori a struttura calcopirite rispetto ai loro analoghi binari sono stimate quantitativamente usando una semplice relazione che implica l’uso della scala delle elettronegatività e il numero quantico principale medio. Il contributo chimico e strutturale a questa anomalia è discusso ed è mostrato che la relazione può essere agevolmente estesa per spiegare la variazione del gap in alcune soluzioni solide.

Резюме Количественно оцениваются аномальные уменьшения ширины запрещенной зоны в полупроводниках халькопирита, по сравнению с бинарными аналогами. Подробно обсуждается химичекий и структурный вклад в эту аномалию. Показывается, что предложенный подход можно легко обобщить для объяснения изменения ширины запрещенной зоны в некоторых кросс-замещенных сплавах.

Summary The anomalous reductions in the energy band gap of the chalcopyrite semiconductors as compared to their relative binary analogs are estimated quantitatively by using a simple relation that involves quantum defect electronegativity scale and the average principal quantum number. The chemical and the structural contribution to this anomaly is discussed in details and it is shown that the relation can be easily extended to explain the variation in the band gap of some cross-substitutional alloys.

Electronic Resource

1432-0738

Glucose ; Glycogen ; Cholinesterase ; Adrenals ; Phosphorylase ; Hexokinase ; Glucose-6-phosphate dehydrogenase

Springer Online Journal Archives 1860-2000

Medicine

Abstract Treatment with diazinon resulted in hyperglycaemia and depletion of glycogen from cerebral and peripheral tissues 2 h after its administration in rats; the changes were maximal after 40 mg/kg diazinon, administered intraperitoneally. The activities of glycogen phosphorylase and phosphoglucomutase were significantly increased in brain and liver, while that of glucose-6-phosphatase was not altered. The activities of the glycolytic enzymes hexokinase and lactate dehydrogenase were increased only in brain. The cholinesterase activity of the brain was reduced by treatment with diazinon. The activities of hepatic gluconeogenic enzymes (fructose 1,6 diphosphatase and phosphoenolpyruvate carboxykinase) were also significantly increased in diazinon-treated animals. The level of lactate was increased in brain and blood while that of pyruvate was not changed. The activity of glucose-6-phosphate dehydrogenase was not significantly changed. Cholesterol and ascorbic acid contents of adrenals were depleted in diazinon-treated animals. Adrenalectomy abolished the hyperglycaemia and changes in carbohydrate metabolism, suggesting the possible involvement of adrenals in the induced changes in diazinon-treated animals.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Monoclonal antibodies (MCAbs) were used to dissect the antigenic sites of the surface glycoproteins of the prototype cell-adapted Wyoming strain of equine infectious anemia virus (EIAV) Serologic reactivities of these MCAbs were determined by ELISA, additive ELISA, competitive ELISA, and Western blot assays. The results indicated that antigenic reactivity of gp90 was localized on at least four distinct epitopes, two of which were important in neutralization. Our studies also revealed that these epitopes were localized on overlapping antigenic sites on gp90. On the other hand, only two distinct non-overlapping epitopes were identified on gp45. Competitive binding studies of neutralizing MCAbs and reference EIA-positive horse serum delineated the presence of a neutralization domain on gp90 that appears to be immunodominant both in naturally infected horses and in mice immunized with EIAV. Limited proteolytic fragmentation of the gp90 component of several serologically distinct EIAV isolates produced common 12K immunoreactive fragments that contained a conserved epitope. These results indicate the occurrence of conserved antigenic regions on EIAV glycoproteins as well as a neutralization domain on gp90, which can be used as potential targets for vaccine development.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary The polypeptide profile of the cell-adapted strain of bovine coronavirus (Mebus BCV-L9) is remarkably affected by the host cell and trypsin. We compared the structural proteins of virus purified from different cell lines and found cell-dependent differences in the virus structure. BCV was purified from four clones of human rectal tumour cells (HRT-18): 3 F3, D 2, 3 E 3, and 4 B 3. The structural profiles of BCV propagated in clones 3 E 3 and 3 F 3 were identical, consisting of proteins with molecular weights of 185, 160, 140, 125, 110, 100, 52, 46, 37, 31–34, and 26–28 kilodaltons (kd). BCV purified from clone D2 lacked the 100 kd species, and clone 4 B 3 yielded virus lacking the 46 kd protein. We compared the structures of BCV propagated in HRT-18 cells [BCV(HRT-18)] and virus raised in bovine fetal spleen cells [BCV(D 2 BFS)]. The concentration of the 185 kd protein was higher in BCV (D 2BFS), and it also contained a 200 kd species. Protein profiles of in vitro trypsin treated and untreated BCV(HRT-18) differed only under reducing conditions, suggesting that trypsin cleavage sites are located within disulfide-linked regions of affected proteins. Propagation of BCV in D 2 BFS cells in the presence of trypsin resulted in cleavage of the 185 kd protein and a concommitant increase of the 100 kd protein. Activation of the fusion function probably depends on this cleavage process because fusion of BCV-infected D 2 BFS cells is trypsin dependent.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary A neutralization escape mutant (A/1 E) of equine infectious anemia virus was isolated after 13 passages in cell culture in the presence of serum containing antibodies to type- and group-specific determinants of EIAV envelope glycoproteins. Loss of neutralization by the selecting serum correlated with loss of two epitopes in the major envelope glycoprotein gp90 of A/1 E which were present in a parallel variant isolated from a persistently infected pony.

Electronic Resource

1572-8943

Springer Online Journal Archives 1860-2000

Chemistry and Pharmacology

Zusammenfassung Chrom(III)-oxid kehrt die Reihenfolge der Dehydratisierung von Bariumperchlorat-Trihydrat (BP) um. Das Oxid reagiert zwischen 223 und 310

РЕжУМЕ ОкИсь тРЕхВАлЕНтНОг О хРОМА ИжМЕНьЕт хОД ДЕгИДРАтАцИИ тРЕхгИ ДРАтИРОВАННОгО пЕРхлОРАтА БАРИь. В ОБ лАстИ тЕМпЕРАтУР 223–310

Abstract Chromium(III) oxide reverses the sequence of dehydration of barium perchlorate trihydrate (BP). Between 223 and 310

Electronic Resource