Search Results - (Author, Cooperation:K. Hosaka)
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1Latest Papers from Table of Contents or Articles in PressL. Tedersoo ; M. Bahram ; S. Polme ; U. Koljalg ; N. S. Yorou ; R. Wijesundera ; L. Villarreal Ruiz ; A. M. Vasco-Palacios ; P. Q. Thu ; A. Suija ; M. E. Smith ; C. Sharp ; E. Saluveer ; A. Saitta ; M. Rosas ; T. Riit ; D. Ratkowsky ; K. Pritsch ; K. Poldmaa ; M. Piepenbring ; C. Phosri ; M. Peterson ; K. Parts ; K. Partel ; E. Otsing ; E. Nouhra ; A. L. Njouonkou ; R. H. Nilsson ; L. N. Morgado ; J. Mayor ; T. W. May ; L. Majuakim ; D. J. Lodge ; S. S. Lee ; K. H. Larsson ; P. Kohout ; K. Hosaka ; I. Hiiesalu ; T. W. Henkel ; H. Harend ; L. D. Guo ; A. Greslebin ; G. Grelet ; J. Geml ; G. Gates ; W. Dunstan ; C. Dunk ; R. Drenkhan ; J. Dearnaley ; A. De Kesel ; T. Dang ; X. Chen ; F. Buegger ; F. Q. Brearley ; G. Bonito ; S. Anslan ; S. Abell ; K. Abarenkov
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-11-29Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: DNA Barcoding, Taxonomic ; Forests ; Fungi/*classification/genetics/*physiology ; Geography ; Grassland ; *Soil ; *Soil Microbiology ; TundraPublished by: -
2Latest Papers from Table of Contents or Articles in PressSeki, T., Hosaka, K., Fischer, C., Lim, S., Andersson, P., Abe, M., Iwamoto, H., Gao, Y., Wang, X., Fong, G.-H., Cao, Y.
Rockefeller University Press
Published 2018Staff ViewPublication Date: 2018-02-10Publisher: Rockefeller University PressPrint ISSN: 0022-1007Electronic ISSN: 1540-9538Topics: MedicineKeywords: MetabolismPublished by: -
3Articles: DFG German National LicensesFujii, Y ; T, Shimada ; Koitabashi, A ; Hashimoto, T ; Hosaka, K ; Tabei, K ; Namatame, T ; Yoneda, M ; Hiraishi, H ; Terano, A
Oxford, UK; Malden, USA : Blackwell Science Inc
Published 2005Staff ViewISSN: 1524-475XSource: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Aim: Trefoil factor family (TFF) peptides are known to facilitate wound healing in gastric mucosa. However, the regulatory mechanisms of gastric TFF expression are not fully understood yet. In this study, we examined the effect of TNF-α on TFF1 and TFF2 expression in gastric epithelial cells.Methods: MKN45 cells were used. TFF mRNA expression was analyzed by real-time quantitative RT-PCR. Promoter sequences of TFF1 gene (−956 to +36) and TFF2 gene (−912 to +24) were inserted into pGL3 vector and reporter gene assays were performed. NF-κB activity was monitored by using a NF-κB responsive element-driven reporter vector.Results: (1) TNF-α(0.1–30 ng/ml) down-regulated TFF1 and TFF2 mRNA expression in a dose-dependent manner. (2) Reporter gene assays also confirmed the down-regulation of TFF1 and TFF2 gene transcription by TNF-α. (3) TNF-α activated NF-κB. (4) Overexpression of dominant negative IκBα prevented both TNF-α-induced NF-κB activation and TNF-α-induced down-regulation of TFF expression.Conclusions: TNF-α down-regulates gastric TFF expression through NF-κB pathway, suggesting that TFF expression is sensitive to inflammatory stimuli.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesMurakami, A. ; Hosaka, K. ; Fukushima, M. ; Tsuya, N.
[S.l.] : American Institute of Physics (AIP)
Published 1988Staff ViewISSN: 1089-7550Source: AIP Digital ArchiveTopics: PhysicsNotes: A digitizer was developed based on the operating principle of electromagnetic induction phenomenon and using high permeability amorphous wires as magnetic cores. The cordless advantage was realized by using a permanent magnet in the stylus pen. A high working height and high resolution of the stylus pen were achieved.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: We previously isolated two distinct Saccharomyces cerevisiae myo-inositol transporter genes, ITR1 and ITR2 (Nikawa et al., 1991). Here, we studied the regulation of their expression by measuring steady-state mRNA levels and β-galactosidase activities of lacZ fusion genes under different conditions. The results show that the expression of the two ITR genes is differently regulated: ITR1 was repressed by inositol and choline whereas ITR2 was constitutive. Deletion analysis of the ITR1 upstream region and comparison with the upstream regions of other genes involved in phospholipid synthesis indicate that the octamer sequence 5′-TTCACATG-3′ is important for the expression and inositol/choline regulation of the ITR1 gene.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: The expression of many genes of Saccharomyces cerevisiae, such as ITR1, is regulated by inositol and choline. In this work, a yeast strain has been constructed in which HIS3 expression is controlled by the ITR1 promoter. Using this strain, three genes were isolated which, when Introduced as multicopies, abolish the repression caused by inositol via the ITR1 promoter. Northern blot analysis revealed that two of these three genes, designated as DIE1 and DIE2, clearly increased the expression of ITR1. DIE2 is more effective for ITR1 expression than DIE1. Gene-disruption experiments revealed that DIE1 was essential for the expression of ITR1 but that DIE2 was not. The sequence of the DIE1 gene was shown to be identical to that of INO2 (also called SCS1), which encodes a protein required for the expression of INO1. DIE2 is a new gene and is capable of encoding 525 amino acid residues with a calculated molecular weight of 61 789. Experiments involving lacZfusion genes showed that multicopy DIE2 resulted in an increase in the expression of both ITR1 and INO1. These results strongly suggest that the DIE1 and DIE2 gene products have an important regulatory function for gene expression of not only ITR1 but also INO1.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesKoitabashi, A. ; Shimada, T. ; Fujii, Y. ; Hashimoto, T. ; Hosaka, K. ; Tabei, K. ; Namatame, T. ; Yoneda, M. ; Hiraishi, H. ; Terano, A.
Oxford, UK : Blackwell Publishing Ltd
Published 2004Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background : Trefoil factor family peptides are expressed in gastrointestinal epithelial cells and play a critical role in maintaining mucosal integrity. Although non-steroidal anti-inflammatory drugs (NSAIDs) are important causative agents of gastric mucosal lesions, few data are available about the effect of NSAIDs on trefoil family peptides in gastric mucosa.Aim : To examine whether indometacin, a widely used NSAID, affects trefoil factor family expression in gastric epithelial cells.Methods : MKN45, a cell line derived from human gastric cancer, was used. TFF1, TFF2, and TFF3 mRNA expression was assessed by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR). TFF2 gene transcription was also examined by luciferase reporter gene assay.Results : Relative expression level of TFF1, TFF2, TFF3 mRNA was 616: 12: 1 in unstimulated MKN45 cells. Although indometacin (1–250 µmol/L) had no significant effect on the expression of TFF1 and TFF3 mRNA, it up-regulated TFF2 mRNA expression in a dose- and time-dependent manner. Luciferase reporter gene assay confirmed the up-regulation of TFF2 gene transcription by indometacin. Indometacin-induced up-regulation of TFF2 expression was not antagonized by externally applied prostaglandin E2.Conclusion : These results suggest that indometacin up-regulates gastric epithelial cell TFF2 expression through a COX-independent mechanism. Since TFF peptides play an important role in gastric mucosal protection, indometacin-induced TFF2 may reduce the degree of gastric mucosal damage induced by indometacin.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesMori, H. ; Morikawa, S. ; Taniguchi, S. ; Fujii, K. ; Fujiwara, M. ; Hosaka, K.
Amsterdam : ElsevierStaff ViewISSN: 0005-2760Keywords: (Bovine retina) ; Base exchange reaction ; Phosphatidylcholine ; Phosphatidylethanolamine ; Phosphatidylserine ; Phospholipase DSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0005-2787Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0003-2697Keywords: acyl-CoA synthetase ; diazocoupling ; free fatty acids ; hydroxamic acid ; iodine ; ureaseSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0014-5793Keywords: Functional cloning ; Human choline kinase cDNA ; Yeast mutantSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0014-5793Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0305-0491Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0305-0491Keywords: Albumin-like protein ; Cyprinus carpio ; carp serum protein ; fastest-moving component ; serum albumin ; serum albumin-like proteinSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 0040-4039Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0040-4039Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 0040-4039Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0040-4039Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 1432-0614Source: Springer Online Journal Archives 1860-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract This study investigates the effects of shear stress on photosynthesis in dilute suspensions of Spirulina platensis and Chlorella by measuring the oxygen production rate using a coaxial, double-rotating-cylinder apparatus that generates Couette shear flow. Our device enables up to 0.6 Pa shear stress to be applied, which has the hydrodynamic effect of generating the algal motion and acutely augmenting the oxygen production rate of Spirulina, primarily because the surface area of algae exposed to illumination is increased. However, there is shear-flow limitation on any increase in oxygen production, and the shear stress at maximum oxygen production rate tends to decrease with increasing temperature. The comparative study with Chlorella showed the reverse relationship between oxygen production and shear stress, and the cause of this difference is discussed in terms of several factors such as size, shape, hydrodynamic stress capacity and others.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 1432-0614Source: Springer Online Journal Archives 1860-2000Topics: BiologyProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract This study investigates the effects of shear stress on photosynthesis in dilute suspensions of Spirulina platensis and Chlorella by measuring the oxygen production rate using a coaxial, double-rotating-cylinder apparatus that generates Couette shear flow. Our device enables up to 0.6 Pa shear stress to be applied, which has the hydrodynamic effect of generating the algal motion and acutely augmenting the oxygen production rate of Spirulina, primarily because the surface area of algae exposed to illumination is increased. However, there is shear-flow limitation on any increase in oxygen production, and the shear stress at maximum oxygen production rate tends to decrease with increasing temperature. The comparative study with Chlorella showed the reverse relationship between oxygen production and shear stress, and the cause of this difference is discussed in terms of several factors such as size, shape, hydrodynamic stress capacity and others.Type of Medium: Electronic ResourceURL: