Search Results - (Author, Cooperation:H. Veelken)

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  1. 1
    Staff View
    Publication Date:
    2012-08-14
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Amino Acid Motifs ; Autoantigens/immunology/metabolism ; Calcium Signaling ; Complementarity Determining Regions/immunology/metabolism ; Epitopes, B-Lymphocyte/immunology/metabolism ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/immunology/*metabolism/*pathology ; Receptors, Antigen, B-Cell/immunology/*metabolism ; *Signal Transduction
    Published by:
    Latest Papers from Table of Contents or Articles in Press
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  3. 3
    Staff View
    ISSN:
    1432-1440
    Keywords:
    Key words Plasmid vector ; Lipofection ; Transgene expression ; Gene therapy ; Nontranscribed Spacer
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract  We evaluated the survival, transgene production, and copy numbers of integrated plasmid units per host genome after lipofection with mono- and bicistronic plasmid vectors in different cell lines and under various conditions. The addition of an integration enhancing murine sequence nontranscribed spacer (NTS) to the plasmids increased transfection efficiency, survival, and transgene expression. However, in human fibroblast cells this sequence had only marginal effects on overall plasmid copy number in bulk cultures. Clones producing the highest amounts of the transgene contained only one or two copies of plasmid per genome, independent of cell type and plasmid design.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
  5. 5
    v. Kalle, Ch. ; Veelken, H. ; Rosenthal, F. M.
    Springer
    Published 1999
    Staff View
    ISSN:
    1433-0415
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Die Gentherapie in der Onkologie verfolgt derzeit Ansätze, die ihre therapeutische Wirkung entweder durch eine direkte Korrektur genetischer Defekte in Tumorzellen erreichen, eine antiproliferative Wirkung, Apoptose oder Suizid in Tumorzellen auslösen, eine Immunantwort gegen Tumorzellen induzieren oder konventionelle Therapieverfahren komplementieren.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
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