Search Results - (Author, Cooperation:D. Milbourne)
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1Latest Papers from Table of Contents or Articles in PressX. Xu ; S. Pan ; S. Cheng ; B. Zhang ; D. Mu ; P. Ni ; G. Zhang ; S. Yang ; R. Li ; J. Wang ; G. Orjeda ; F. Guzman ; M. Torres ; R. Lozano ; O. Ponce ; D. Martinez ; G. De la Cruz ; S. K. Chakrabarti ; V. U. Patil ; K. G. Skryabin ; B. B. Kuznetsov ; N. V. Ravin ; T. V. Kolganova ; A. V. Beletsky ; A. V. Mardanov ; A. Di Genova ; D. M. Bolser ; D. M. Martin ; G. Li ; Y. Yang ; H. Kuang ; Q. Hu ; X. Xiong ; G. J. Bishop ; B. Sagredo ; N. Mejia ; W. Zagorski ; R. Gromadka ; J. Gawor ; P. Szczesny ; S. Huang ; Z. Zhang ; C. Liang ; J. He ; Y. Li ; Y. He ; J. Xu ; Y. Zhang ; B. Xie ; Y. Du ; D. Qu ; M. Bonierbale ; M. Ghislain ; R. Herrera Mdel ; G. Giuliano ; M. Pietrella ; G. Perrotta ; P. Facella ; K. O'Brien ; S. E. Feingold ; L. E. Barreiro ; G. A. Massa ; L. Diambra ; B. R. Whitty ; B. Vaillancourt ; H. Lin ; A. N. Massa ; M. Geoffroy ; S. Lundback ; D. DellaPenna ; C. R. Buell ; S. K. Sharma ; D. F. Marshall ; R. Waugh ; G. J. Bryan ; M. Destefanis ; I. Nagy ; D. Milbourne ; S. J. Thomson ; M. Fiers ; J. M. Jacobs ; K. L. Nielsen ; M. Sonderkaer ; M. Iovene ; G. A. Torres ; J. Jiang ; R. E. Veilleux ; C. W. Bachem ; J. de Boer ; T. Borm ; B. Kloosterman ; H. van Eck ; E. Datema ; B. Hekkert ; A. Goverse ; R. C. van Ham ; R. G. Visser
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-07-12Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Evolution, Molecular ; Gene Duplication ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genetic Variation ; Genome, Plant/*genetics ; *Genomics ; Haplotypes/genetics ; Heterozygote ; Homozygote ; Immunity, Innate ; Inbreeding ; Molecular Sequence Annotation ; Molecular Sequence Data ; Plant Diseases/genetics ; Ploidies ; Solanum tuberosum/*genetics/physiologyPublished by: -
2Articles: DFG German National LicensesLuo, Z. W. ; Hackett, C. A. ; Bradshaw, J. E. ; McNicol, J. W. ; Milbourne, D.
Springer
Published 2000Staff ViewISSN: 1432-2242Keywords: Key words Autotetraploid ; Potato ; Codominant marker ; Genotype prediction ; MappingSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Recent genome mapping projects in tetraploid plant species require a method for analysing the segregation patterns of molecular marker loci in these species. The present study presents a theoretical model and a statistical analysis for predicting the genotypes of a pair of tetraploid parents at a codominant (for example, RFLPs, microsatellites) or dominant (for example, AFLPs, RAPDs) molecular marker locus based on their and their progeny’s phenotypes scored at that locus (gel-band patterns). The theory allows for null alleles and for any degree of double-reduction to be modelled. A simulation study was performed to investigate the properties of the theoretical model. This showed that in many circumstances both the parental genotypes can be correctly identified with a probability of nearly 1, even when the molecular data were complicated by null alleles or double-reduction. Configurations where the parental genotype cannot be identified are discussed. The power to detect double-reduction varies considerably, depending on the proportion of identical alleles carried and shared by the parents, and the number of null alleles. Incorrect deductions of the occurrence of double-reduction were rare. The method was applied to data on a microsatellite locus segregating in the parents and 74 offspring of a tetraploid potato cross. Twentyfour parental configurations were consistent with the parental gel pattern, but only one of these was compatible with all the phenotypic data on the offspring. The feasibility for extending the present model to predict segregation of several linked loci, and particularly the linkage phase, is briefly discussed.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesBradshaw, J. E. ; Hackett, C. A. ; Meyer, R. C. ; Milbourne, D. ; McNicol, J. W. ; Phillips, M. S. ; Waugh, R.
Springer
Published 1998Staff ViewISSN: 1432-2242Keywords: Key words Potato ; Solanum tuberosum ; Nematode resistance ; Globodera pallida ; Linkage in autotetraploids ; Quantitative trait locusSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Seventy eight clones from the cross between SCRI clone 12601ab1 and cv Stirling were used to explore the possibility of genetical linkage analysis in tetraploid potato (Solanum tuberosum subsp. tuberosum). Clone 12601ab1 had quantitative resistance to Globodera pallida Pa2/3 derived from S. tuberosum subsp. andigena. The strategy adopted involved identifying single- (simplex) and double- (duplex) dose AFLP markers in the parents from segregation ratios that could be unambiguously identified in their offspring, detecting linkage between a marker and a putative quantitative trait locus (QTL) for resistance, and placing the QTL on the linkage map of markers. The numbers of scorable segregating markers were 162 simplex ones present only in 12601ab1, 87 present in Stirling, and 32 present in both; and 72 duplex markers present only in 12601ab1 and 45 present in Stirling. The total map length was 990.9 cM in 12601ab1 and 484.6 cM in Stirling. A QTL with a resistance allele present in double dose (QQqq) in 12601ab1 was inferred from the associations between resistance scores (square root of female counts) and two duplex markers linked in coupling, which, in turn, were linked in coupling to four simplex markers also associated with resistance, but to a lesser degree. The largest marker class difference was the one for the duplex marker P61M34=15. It accounted for 27.8% of the phenotypic variance in resistance scores, or approximately 30% of the genotypic variance. Subsequently, this duplex marker was found to be linked in coupling with a duplex SSR allele Stm3016=a, whose locus was shown to be on chromosome IV in a diploid reference mapping population. The other QTLs for resistance segregating in the progeny were not identified for one or more of the following reasons: the markers did not cover the whole of the genome, there were unfavourable repulsion linkages between the QTLs and markers, or the gene effects were not large enough to be detected in an experiment of the size conducted. It is concluded that prospects appear good for detecting QTLs and using marker-assisted selection in a tetraploid potato breeding programme, provided that, in future, the population size is increased to over 250 and more SSR markers are used to complement the AFLPs; the same is likely to be true for other autotetraploid crops.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesCollins, A. ; Milbourne, D. ; Ramsay, L. ; Meyer, R. ; Chatot-Balandras, C. ; Oberhagemann, P. ; De Jong, W. ; Gebhardt, C. ; Bonnel, E. ; Waugh, R.
Springer
Published 1999Staff ViewISSN: 1572-9788Keywords: potato ; late blight ; QTL ; Linkage mapping ; earliness ; vigourSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract Field resistance to Phytophthora infestans, the causal agent of foliage and tuber blight in cultivated potatoes, earliness (maturity) and vigour, were examined in a diploid segregating potato population grown in replicated trials over three consecutive growing seasons. A genetic linkage map of this population was constructed in parallel using PCR-based SSR, AFLP and CAPS markers. Analysis of the trait scores alongside the marker segregation data allowed the identification of regions of the genome which were significantly correlated with components of the respective characters. The most significant associations for all four traits were with marker alleles on potato linkage group V originating from the male (susceptible) parent. In the case of foliage resistance to late blight, the positions of the majority of the effects, which were located on eleven of the twelve potato linkage groups, have been detected in previous [16] and parallel studies [21]. The absence of Solanum demissum-derived R genes for hypersensitive response to late blight and the co-localisation of QTL for resistance, vigour and earliness suggest that developmental and/or physiological factors play a major role in determining the level of foliage resistance in this population. In contrast with previous findings, a negative correlation was found between foliage and tuber blight resistance.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesMilbourne, D. ; Meyer, R. C. ; Collins, A. J. ; Ramsay, L. D. ; Gebhardt, C. ; Waugh, R.
Springer
Published 1998Staff ViewISSN: 1617-4623Keywords: Key words Simple sequence repeat ; Linkage map ; Single-strand hybridisation ; Triplex affinity capture ; Enriched librarySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Solanum tuberosum L. DNA sequences containing simple sequence repeat (SSR) motifs were extracted from the EMBL database, cDNA and selectively enriched small-insert DNA libraries. Enrichment was achieved using either triplex affinity capture or single-strand hybridisation selection. One hundred and twelve primer pairs which successfully amplified products of the correct size from potato DNA were ultimately designed and synthesised. Ninety-eight of these revealed length polymorphisms in a panel of four diploid and two tetraploid clones, in agreement with the high information content of this class of markers which has been found in other species. All of the markers were assigned a quality score of 1–5 based on their potential usefulness. Eighty-nine loci from 65 of the primer pairs were located on two genetic linkage maps of potato by segregation analysis of the amplified alleles. Fifty-two of the SSRs were clearly single locus. The maps were aligned using 23 SSR primer pairs and 13 RFLP loci mapped in both populations. The markers described constitute a class which should replace Restriction Fragment Length Polymorphisms (RFLP) as the markers of choice for future genetic studies in potato. The sequences of the primers, together with other information on these markers are provided.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesMeyer, R. C. ; Milbourne, D. ; Hackett, C. A. ; Bradshaw, J. E. ; McNichol, J. W. ; Waugh, R.
Springer
Published 1998Staff ViewISSN: 1617-4623Keywords: Key words AFLP ; Linkage map ; Solanum tuberosumSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract We have constructed a partial linkage map in tetraploid potato which integrates simplex, duplex and double-simplex AFLP markers. The map consists of 231 maternal and 106 paternal markers with total map lengths of 990.9 cM and 484.6 cM. The longer of the two cumulative map lengths represents approximately 25% coverage of the genome. In tetraploids, much of the polymorphism between parental clones is masked by `dosage' which significantly reduces the number of individual markers that can be scored in a population. Consequently, the major advantage of using AFLPs – their high multiplex ratio – is reduced to the point where the use of alternative multi-allelic marker types would be significantly more efficient. The segregation data and map information have been used in a QTL analysis of late blight resistance, and a multi-allelic locus at the proximal end of chromosome VIII has been identified which contributes significantly to the expression of resistance. No late blight resistance genes or QTLs have previously been mapped to this location.Type of Medium: Electronic ResourceURL: