Search Results - (Author, Cooperation:D. L. Barber)

2015-01-17

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Adaptive Immunity ; Animals ; Antibodies, Viral/immunology ; Antigens, Viral/immunology ; Arenaviridae Infections/*immunology/virology ; CD4-Positive T-Lymphocytes/*immunology ; CD8-Positive T-Lymphocytes/immunology ; Cytokines/blood ; Epitopes, T-Lymphocyte/immunology ; Immune System Diseases/*etiology/immunology/pathology ; Immunologic Memory ; Inflammation/*etiology/immunology/pathology ; Lymphocytic choriomeningitis virus/*immunology/physiology ; Mice, Inbred C57BL ; Multiple Organ Failure/etiology ; Vaccination ; Viral Load ; Viral Vaccines/*adverse effects/*immunology ; Virus Replication

2012-07-06

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Aging ; Animals ; Bone Marrow/pathology ; Cell Lineage ; CpG Islands/genetics ; DNA Methylation ; Disease Models, Animal ; Epigenesis, Genetic/*genetics ; Female ; Gene Knock-In Techniques ; Glioma/pathology ; Hematopoiesis ; Hematopoietic Stem Cells/*cytology/metabolism ; Histones/metabolism ; Humans ; Isocitrate Dehydrogenase/*genetics/*metabolism ; Leukemia, Myeloid, Acute/genetics ; Male ; Mice ; Mutant Proteins/genetics/*metabolism ; Mutation/*genetics ; Myeloid Cells/cytology/metabolism ; Spleen/pathology

2015-05-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Enzyme Activation ; Humans ; Microscopy, Electron, Transmission ; *Models, Molecular ; Mutation/genetics ; Neoplasms/*enzymology/genetics ; Phosphofructokinase-1/*chemistry/*genetics/ultrastructure ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry/genetics/metabolism

2014-07-06

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Dinoprostone/antagonists & inhibitors/biosynthesis/metabolism ; Disease Models, Animal ; Female ; Humans ; Immunity, Innate/immunology ; *Immunotherapy ; Interferon Type I/antagonists & inhibitors/biosynthesis/*immunology ; Interleukin-1/*immunology ; Male ; Mice ; Mice, Inbred C57BL ; Mycobacterium tuberculosis/*immunology ; Tuberculosis, Pulmonary/*immunology/microbiology/*therapy

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Light and electron microscopic studies of the morphological features of immature and mature rodlet cells in Catostomus commersoni are presented emphasizing the cells' association with epithelial tissues. The peripheral fibrillar layer is lacking from the apex and from the base of the cell. A cytoplasmic extension from the base may be a feeding mechanism whereby the rodlet cell obtains nutrient at the expense of adjacent cells leaving intercellular spaces often containing myelin figures. RNAase digestion studies demonstrate the presence of RNA in the electron dense rodlet core.The structure and histochemistry of the rodlets which do not appear to disintegrate upon expulsion from the cell are compared to the cytoplasmic inclusions of both normal fish cells and protozoan parasites. The possible association of the rodlet cell with various pathological conditions is briefly reviewed and the authors conclude that it is premature to disregard the possibility that this cell could be a parasite or infective agent.

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

DNA of rodlet cells and erythrocytes from three species of freshwater teleosts, Semotilus atromaculatus atromaculatus, Catostomus commersoni and Cyprinus carpio, was stained with the Feulgen reaction and examined by microdensitometry. Rodlet cells showed nuclear DNA content significantly different from erythrocytes of the same species, but the difference was less than a factor of C, assuming that erythrocytes reflect the normal 2C genome of somatic cells. In two species, S. atromaculatus and C. carpio, the rodlet cell nuclei contained less DNA than the erythrocytes; in C. commersoni they contained more. The identity of the rodlet cell is unknown; the results of these experiments lead to the rejection of the hypothesis that rodlet cells and erythrocytes of a species have the same DNA content, i.e. that the rodlet cell is a normal somatic component of fish tissue.

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Peripheral blood and haemopoietic tissues of spleen and kidney of the icefish, Chaenocephalus aceratus were examined using LM and EM techniques. The peripheral blood contained cellular elements from all the recognized cell lines usually seen in other teleost groups. Erythrocytes were very rare; when found, they were mature or senile and fragile. Thrombocytes of two morphologies, several cell types considered to be part of the lymphoid series and monocytes/macrophages were present. Two distinctive types of granulocytes also were found; their morphologies and granulation were so different from teleost granulocytes hitherto described that their identification was impossible.

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Nuclear DNA contents of rodlet cells from Catostomus commersoni, Semotilus atromaculatus and Cyprinus carpio were compared with nuclear DNA of erythrocytes and larger cells of the same species, using scanning microdensitometry and averaging microdensitometry. This study reappraises the work of Barber & Westermann (1983), which employed averaging microdensitometry only, and compared rodlet cell nuclear DNA only with erythrocyte DNA. In addition, this work considers sources of error in both methods of microdensitometry, and comments upon the use of microdensitometry of either method as a mechanism for making distinctions among the DNA contents of cells of different types. The results of the present work consistently indiate no significant differences within species between nuclear DNA content of rodlet cells and larger teleost cells, using either method of microdensitometry. Because of the lack of statistically significant difference in DNA content between nuclei of rodlet cells and those of known teleost cells, it has been concluded that the rodlet cell itself is probably of teleost origin. However, the method indicates nothing about the origin of the rodlets, which have also been shown to contain DNA, but are Feulgen-negative.

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Developmental stages of the two circulating blood granulocytes, the heterophil and the periodic acid-Schiff-positive granular leukocyte (PAS-GL) are described. There are no metachromatic basophils in this fish and eosinophils, when present, are confined to extra-vascular tissues. Fish were killed up to 24 h after injection with histamine liberator 48/80. Morphological (L/M and E/M) and histochemical studies of blood, haemopoietic tissue and gills from experimental fish were compared with those of untreated control fish.Blood cells other than PAS-GL and heterophils were generally unaffected by 48/80. Most PAS-GL and heterophils showed alterations in granules and other cytoplasmic features. Stretching and splitting of the PAS-GL nucleus occurred. Very little, if any, histamine was detectable in PAS-GL and heterophils.The authors support the postulate that during evolution PAS-GL become basophils/ mast cells through two major stages, (1) sulphation of the granule polysaccharide to give heparin resulting in metachromasia of the granules and (2) storage of large quantities of histamine in the granules.

Electronic Resource

1573-5192

Springer Online Journal Archives 1860-2000

Biology

Medicine

Abstract A new species of haemogregarina, Haemogregarina nototheniae, is described from the Southern ocean teleosts Notothenia neglecta and Notothenia rossii. Stages identified as macro- and microschizogony and gametogony are described in mononuclear leukocytes from fish caught during the austral summer. The mature gametocyte is the most commonly found stage: it is exoerythrocytic, but carries the host erythrocyte nucleus attached to its external surface near one end. The gametocyte has a central nucleus and 2–16 subterminal eosinophilic granules, but no polar cap. During microschizogony the schizont nucleus undergoes repeated division without cytoplasmic division to give 32 nuclear masses, all of which appear to be in metaphase. Cytoplasmic division yields free merozoites identifiable by the coarse chromatin of the nuclear area. During macroschizogony the intraleukocytic parasite swells to a subspherical mass with a median band of fine heterochromatin granules. The cytoplasm later divides, forming three merozoites. There appear to be two routes by which merozoites proceed to become gametocytes: in winter small merozoites are seen in mature erythrocytes; but in summer, in erythroblasts. The invertebrate definitive host and the means of transmission are unknown, but the parasite is provisionally assigned to the genus Haemogregarina.

Electronic Resource

1573-5192

Springer Online Journal Archives 1860-2000

Biology

Medicine

Abstract Haemogregarina georgianae n. sp. occurs frequently in the bathydraconid teleost Parachaenichthys georgianus from the western Antarctic portion of the Southern Ocean. The haemogregarine and its developmental stages in the vertebrate have been found in erythrocytes of the fish: both microschizogony and macroschizogony have been seen in fish caught during the austral summer. Morphological evidence suggests the merozoites from macroschizogony give rise to the microschizont, and that the microschizont merozoites give rise to gametocytes. Comparison of H. georgianae with other haemogregarines known from teleosts shows that it has a previously undescribed morphology.

Electronic Resource