Search Results - (Author, Cooperation:D. Gilmour)

2013-09-27

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Cell Movement/*physiology ; Chemokine CXCL12/genetics/metabolism ; Chemotactic Factors/genetics/*metabolism ; Embryo, Nonmammalian ; Gene Expression Regulation, Developmental ; Receptors, CXCR/genetics/metabolism ; Zebrafish/genetics/*physiology ; Zebrafish Proteins/genetics/metabolism

2014-10-23

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; *Cell Communication ; Cell Differentiation ; Cell Movement ; Dose-Response Relationship, Drug ; Extracellular Space/metabolism ; Fibroblast Growth Factors/metabolism/secretion ; *Organogenesis ; *Signal Transduction ; Time Factors ; Zebrafish/*embryology/metabolism

2018-01-30

The American Society for Microbiology (ASM)

0270-7306

1098-5549

Biology

Medicine

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

In-vitro-cultured bursal epithelium (BE) and BE-conditioned medium (BECM) induce B-L antigen on chicken intraembryonic cells. Cells prepared from 9-day-old intraembryonic mesoderm were fractionated in accordance with cell size by linear albumin gradient sedimentation at 1 g. Two cell types could be distinguished on which expression of the B-L antigen was altered after a 6-h incubation with the bursal epithelial component. One fraction contained small mononuclear cells with low sedimentation velocity (≤ 3 mm/h) and low spontaneous proliferation activity. These cells responded strongly to BECM and showed a slight but not significant response to BE (index after incubation with BECM 3.8, with BE 1.7, as compared with RPMI medium control). The other fraction was composed of large mononuclear cells with sedimentation velocity 〉 9 mm/h and with high spontaneous proliferation. These cells showed a response of equal magnitude to both BE and BECM (index after incubation with BE 2.0, with BECM 2.3). These results suggest that the bursa of Fabricius has influence on two different cell types: a large, probably primitive undifferentiated cell, responding equally to bursal cellular contacts and BE culture supernatant, and a small mononuclear cell type, probably more mature, responding more clearly to the bursal humoral factor.

Electronic Resource

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Allogeneic yolk sac—embryo chimaeras were constructed by association of S15B15 yolk sac and B2B2 embryo on day 2 of incubation. Five days later yolk sac cells from the chimaeras were injected intravenously into 14-day-old irradiated embryos, using recipients of B2B2 and B15B15 genotypes. One week after hatching, cells in the bursa of Fabricius and peripheral blood erythrocytes were studied for la-like antigens and B alloantigens, respectively, to determine whether they were derived from the embryo or yolk sac part of the chimaera. The results obtained demonstrate that prebursal and erythropoietic stem cells migrate from the early embryo to the yolk sac during the 2nd to the 7th day of incubation. They also exclude the de novo generation of prebursal stem cells in the yolk sac.

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] Fig. 1. Starch-gel electrophoresis of cock sera, showing the three transferrin types. Y Y marks the position of insertion of the samples, and X X the position of the brown line at the end of the run In order to avoid possible confusion owing to the known variation dependent on egg-laying, most of ...

Electronic Resource

0022-1910

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0003-9861

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] (1) Heat wactivation. Sera containing complete agglutinins were diluted 1/4 and then heated for 10 min. at 70 C. The heated sera were then tested against the appropriate cells in saline, by an albumin replacement technique2 and by a spin antiglobulin technique using rabbit anti-chicken serum. The ...

Electronic Resource

1432-1238

Transport ; Mobile Intensive Care Unit ; Specialised Investigation ; Ultrasound ; Pulmonary Angiography ; Sepsis

Springer Online Journal Archives 1860-2000

Medicine

Abtract Critically ill patients can be safely moved within hospital using a mobile intensive care unit (MICU). The MICU allows the critically ill to benefit from specialised investigation and treatment they might otherwise be denied. The MICU in use at the Western Infirmary, Glasgow is described and its merits outlined in the light of clinical experience gained over a twelve month period.

Electronic Resource

1432-2048

Chlorophyll fluorescence ; Dunaliella (osmoregulation) ; Osmoregulation ; Photosynthesis (ionic stress)

Springer Online Journal Archives 1860-2000

Biology

Abstract A comparison of the effects of ionic stress and an uncoupler on long-term fluorescence transients (the ‘Kautsky effect’) in the green alga Dunaliella tertiolecta indicated that the large quenching induced by ionic stress was caused by a pH gradient across the thylakoid membrane. This possiblity was given support by the increase in the slow phase of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea-induced fluorescence relaxation in algae subjected to ionic stress. Low-temperature fluorescence emission spectra indicated that salt stress enhanced photosystem-I emission in the dark, and a comparison of simultaneous emissions at 695 and 720 nm at room temperature indicated a further increase in photosystem-I emission during the fluorescence transients. Taken together with the decrease in the fast phase of 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea-induced fluorescence relaxation in stressed algae, our results indicate that ionic stress stimulates cyclic electron flow, and that non-cyclic flow is inhibited. The effect of sucrose-induced osmotic stress was similar to, but less marked than, the effects of NaCl and KCl; the effect of decreasing the external salinity was small.

Electronic Resource

1432-072X

Chlorococcum ; Unicellular green alga ; Salt shock ; Intracellular solute concentrations ; Photosynthesis

Springer Online Journal Archives 1860-2000

Biology

Abstract Cell volumes and intracellular concentrations of major solutes of Chlorococcum submarinum were determined before and after salinity shocks. Cells were found to shrink in size by about 30% following changes from 0.1 to 0.5 M NaCl, there was a transitory increase in sodium concentration and more permanent increases in concentrations of potassium, proline and glycerol (the major osmolyte). Conversely, cells doubled in size after the reciprocal downshock, there was rapid loss of about 70% of the cells' glycerol to the medium, a much smaller loss of cellular potassium and a steady disappearance of proline from the cells. The respiratory and photosynthetic responses to salinity fluctuations were also studied. Salinity downshocks stimulated respiration by 30% and inhibited photosynthesis by 16% within 5 min, but within 2 h these rates were identical to control rates. Upshocks caused a slight inhibition of respiration, but decreased photosynthesis by 40% within 5 min and recovery took 2 h. Downshocks had little effect on chlorophyll fluorescence, however, Fo strongly increased and both Fm and Fv/Fm declined within 5 min of salinity increases. This is consistent with a decrease in efficiency of PS2. Ecological and metabolic implications of the results are discussed.

Electronic Resource

1432-072X

Halomonas SPC1 ; Turgor regulation ; Inorganic and organic compatible solute accumulation

Springer Online Journal Archives 1860-2000

Biology

Abstract The mechanisms by which a novel eubacterium, identified as belonging to the genus Halomonas, adapted to increases in the extracellular osmotic potential were investigated. It was shown that the ability of the bacterium to grow after hyperosmotic shock was dependent on the presence of potassium ions. Growth of the bacterium in 2 M NaCl medium could be limited by low concentrations of K+ and this enabled the affinity for K+ to be determined (K s=21.5 μM). Rubidium salts could be substituted for those of potassium, but the lowest concentration of Rb+ that allowed growth in 2 M NaCl medium was 50-fold greater than the minimum concentration of K+. 13C-NMR spectroscopy and HPLC analysis were used to demonstrate the accumulation of organic solutes in the cytoplasm after exposure to high salinities. The major osmolyte was ectoine, but glutamate and ectoine hydroxide were also present. Addition of exogenous glycine betaine to 3.25 M NaCl medium resulted in the accumulation of high intracellular concentrations of glycine betaine in the bacterium. This reduced the level of ectoine accumulation but did not fully inhibit the synthesis of this compound in the cytoplasm.

Electronic Resource

1432-072X

Chlorococcum ; Unicellular green alga ; Cell volume determination ; Glycerol

Springer Online Journal Archives 1860-2000

Biology

Abstract A method is described for measuring the cell volume of the unicellular green alga Chlorococcum submarinum, which depends on measurements of bromide concentration before and after disruption of the cells by ammonium hydroxide. Simultaneous equations are derived, which along with direct determination of cell water weight, allow the calculation of the intracellular volume in three different ways. The volumes calculated are in agreement indicating the validity of the method. The cell volumes and internal concentrations of glycerol, proline, potassium and sodium were determined for algae adapted to three salinities, 0.1, 0.5 and 1.0 M NaCl. The results showed that glycerol was the major internal solute and that the total measured solutes balanced the external osmotic pressure at all three salinities.

Electronic Resource

1432-1211

Springer Online Journal Archives 1860-2000

Biology

Medicine

Electronic Resource

1432-203X

Springer Online Journal Archives 1860-2000

Biology

Abstract Protoplasts isolated from cell suspensions of albinoMedicago borealis andM. sativa were fused chemically, using two methods, and electrically. Although a small scale method of chemical fusion gave the highest fusion frequency, electrofusion was the superior technique on the basis of throughput of green somatic hybrid cell colonies. Chlorophyll-containing tissues were confirmed as being somatic hybrid by isoenzyme and cytological analyses. This is the first report of the application of albino complementation to produce somatic hybrid cells in forage legumes.

Electronic Resource