Search Results - (Author, Cooperation:A. D'Hont)
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1A. D'Hont ; F. Denoeud ; J. M. Aury ; F. C. Baurens ; F. Carreel ; O. Garsmeur ; B. Noel ; S. Bocs ; G. Droc ; M. Rouard ; C. Da Silva ; K. Jabbari ; C. Cardi ; J. Poulain ; M. Souquet ; K. Labadie ; C. Jourda ; J. Lengelle ; M. Rodier-Goud ; A. Alberti ; M. Bernard ; M. Correa ; S. Ayyampalayam ; M. R. McKain ; J. Leebens-Mack ; D. Burgess ; M. Freeling ; A. M. D. Mbeguie ; M. Chabannes ; T. Wicker ; O. Panaud ; J. Barbosa ; E. Hribova ; P. Heslop-Harrison ; R. Habas ; R. Rivallan ; P. Francois ; C. Poiron ; A. Kilian ; D. Burthia ; C. Jenny ; F. Bakry ; S. Brown ; V. Guignon ; G. Kema ; M. Dita ; C. Waalwijk ; S. Joseph ; A. Dievart ; O. Jaillon ; J. Leclercq ; X. Argout ; E. Lyons ; A. Almeida ; M. Jeridi ; J. Dolezel ; N. Roux ; A. M. Risterucci ; J. Weissenbach ; M. Ruiz ; J. C. Glaszmann ; F. Quetier ; N. Yahiaoui ; P. Wincker
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-07-18Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Conserved Sequence/genetics ; DNA Transposable Elements/genetics ; *Evolution, Molecular ; Gene Duplication/genetics ; Genes, Plant/genetics ; Genome, Plant/*genetics ; Genotype ; Haploidy ; Molecular Sequence Data ; Musa/classification/*genetics ; PhylogenyPublished by: -
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ISSN: 0021-9673Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
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ISSN: 0168-9452Keywords: Medicago ; mitochondrial and chloroplastic DNAs ; mitochondrial recombination ; somatic hybridsSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
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ISSN: 1432-2242Keywords: Key words Musa spp ; Banana ; Genomic in situ hybridization ; Aneuploidy ; Polyploidy ; rDNA genesSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract In cultivated banana, Musa spp., there are four known genomes, A, B, S, and T. These correspond to the genetic constitutions of wild Eumusa species M. acuminata, M. balbisiana, M. schizocarpa and the Australimusa species, respectively. Most cultivated clones are triploid or diploid, they have been classified into genomic groups according to chromosome numbers and morphological traits. Genomic in situ hybridization (GISH) enabled us to differentiate the chromosomes of these four genomes; however, a distal portion of the chromosomes remained unlabelled. GISH was used to determine the exact genome structure of interspecific cultivated clones.In most cases the results were consistent with the chromosome constitution estimated by means of phenotypic descriptors.The one notable exception, the clone ’Pelipita’, has the 8 A and 25 B chromosomes instead of the predicted 11 A and 22 B. GISH also enabled us to determine the chromosome complement of a few clones that could not be classified only on the basis of phenotypic descriptors and chromosome counts. The rDNA sites were located in Musa species. They appeared to be often associated with satellites, which can be separated from the chromosomes, representing a potential source of error for chromosome counting using classical techniques.Type of Medium: Electronic ResourceURL: -
5Asnaghi, C. ; Paulet, F. ; Kaye, C. ; Grivet, L. ; Deu, M. ; Glaszmann, J. C. ; D’Hont, A.
Springer
Published 2000Staff ViewISSN: 1432-2242Keywords: Key words Sugarcane ; Rust resistance gene ; Comparative mapping ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A major rust resistance gene has been identified in a self-progeny of the sugarcane cultivar R570. Until now, this gene was known to be linked to a marker revealed by the sugarcane probe CDSR29 but unassigned to any linkage group of the current genetic map. We used synteny relationships between sugarcane and three other grasses in an attempt to saturate the region around this rust resistance gene. Comparison of sugarcane, sorghum, maize and rice genetic maps led to the identification of homoeologous chromosome segments at the extremity of sorghum linkage group D, rice linkage group 2, maize linkage group 4 and in the centromeric region of maize linkage group 5. One hundred and eighty-four heterologous probes were selected and tested for cross-hybridization with sugarcane DNA; 106 produced a good hybridization signal and were hybridized on 88 individuals of the R570 selfed progeny. Two hundred and seventeen single-dose markers were added to the R570 genetic map, of which 66% mapped to linkage group VII, together with the rust resistance gene. This gene has now been mapped to the end of a co-segregating group consisting of 19 RFLP markers. None of the mapped loci were located closer to the gene than CDSR29. The gene thus appears to reside at the edge of a ’’synteny cluster’’ used to describe the different grass genomes.Type of Medium: Electronic ResourceURL: -
6Dufour, P. ; Grivet, L. ; D’Hont, A. ; Deu, M. ; Trouche, G. ; Glaszmann, J. C. ; Hamon, P.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Key words Maize ; Sorghum ; Sugarcane ; RFLP ; SyntenySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Comparative mapping within maize, sorghum and sugarcane has previously revealed the existence of syntenic regions between the crops. In the present study, mapping on the sorghum genome of a set of probes previously located on the maize and sugarcane maps allow a detailed analysis of the relationship between maize chromosomes 3 and 8 and sorghum and sugarcane homoeologous regions. Of 49 loci revealed by 46 (4 sugarcane and 42 maize) polymorphic probes in sorghum, 42 were linked and were assigned to linkage groups G (28), E (10) and I (4). On the basis of common probes, a complete co-linearity is observed between sorghum linkage group G and the two sugarcane linkage groups II and III. The comparison between the consensus sorghum/sugarcane map (G/II/III) and the maps of maize chromosomes 3 and 8 reveals a series of linkage blocks within which gene orders are conserved. These blocks are interspersed with non-homoeologous regions corresponding to the central part of the two maize chromosomes and have been reshuffled, resulting in several inversions in maize compared to sorghum and sugarcane. The results emphasize the fact that duplication will considerably complicate precise comparative mapping at the whole genome scale between maize and other Poaceae.Type of Medium: Electronic ResourceURL: -
7Daugrois, J. H. ; Grivet, L. ; Roques, D. ; Hoarau, J. Y. ; Lombard, H. ; Glaszmann, J. C. ; D'Hont, A.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Major gene ; Polyploidy ; Puccinia melanocephala ; Sugarcane ; RFLP ; RustSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Inheritance of resistance to rust was investigated in the self progeny of the sugarcane cultivar ‘R570’ also used to build a RFLP genetic map. Resistance was evaluated through both field and controlled greenhouse trials. A clear-cut 3 (resistant) ∶ 1 (susceptible) segregation indicative of a probable dominant resistant gene was observed. This is the first documented report of a monogenic inheritance for disease resistance in sugarcane. This gene was found linked at 10 cM with an RFLP marker revealed by probe CDSR29. Other minor factors involved in the resistance were also detected.Type of Medium: Electronic ResourceURL: -
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ISSN: 1432-2242Keywords: Key words Sugarcane cultivars ; Linkage disequilibrium ; Foundation bottleneck ; Interspecific hybrids ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Modern sugarcane cultivars are derived from a few interspecific hybrids created early in this century. Linkage disequilibrium was investigated in a population of 59 cultivars representing the most important commercial clones bred in Mauritius as well as a few old cultivars involved in their genealogy. Thirty-eight probes scattered over the sugarcane genome map were used to reveal RFLPs. Forty-two cases of bilocus associations were observed involving a total of 33 loci. Most of them are separated by less than 10 cM. All the corresponding allele couples were found in at least 1 of the originally created cultivars, suggesting that they depict ancient associations. This global disequilibrium is interpreted as the result of the foundation bottleneck related to the first interspecific crosses; the preferential allele associations thus created have been maintained through subsequent crosses when the loci were closely linked. This phenomenon is likely also to apply to genes of agricultural interest. A practical consequence is that markers can be used to track known QTLs in modern breeding materials without the necessity to repeatedly study segregating progenies. This structure gives high value to the correlation between molecular markers and agricultural traits among cultivars.Type of Medium: Electronic ResourceURL: -
9Staff View
ISSN: 1432-2242Keywords: Key words Alu-like sequences ; Repeated DNA ; Species-specific sequences ; Saccharum complex ; SurgarcaneSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Alu sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The Alu-PCR method, which consists of amplification between Alu sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of Alu-like sequences in sugarcane and related species by applying the Alu-PCR-like method. Amplifications using a PCR primer defined in conserved regions of Alu human sequences lead to specific complex multiband profiles in all the Saccharum and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific inter-Alu-like fragments allowed us to isolate repeated sequences that are specific for different genera of the Saccharum complex: MsCIR2 from Miscanthus, EaCIR6 and EaCIR7 from Erianthus, and SrCIR2 from Saccharum. Two PCR diagnostic tests were developed from the inter-Alu-like sequences MsCIR2 and EaCIR6, and proved efficient in identifying intergeneric hybrids Saccharum×Miscanthus or Saccharum×Erianthus, respectively. The present study illustrates how the Alu-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants.Type of Medium: Electronic ResourceURL: -
10D'Hont, A. ; Rao, P. S. ; Feldmann, P. ; Grivet, L. ; Islam-Faridi, N. ; Taylor, P. ; Glaszmann, J. C.
Springer
Published 1995Staff ViewISSN: 1432-2242Keywords: Saccharum ; Intergeneric hybrids ; Isozymes ; RFLP ; STS-PCR ; In situ hybridization ; rDNASource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Molecular markers were used to characterise sugarcane intergeneric hybrids between S. officinarum and E. arundinaceus. Very simple diagnostic tools for hybrid identification among the progeny were derived from isozyme electrophoresis and a sequence-tagged PCR. Two enzyme systems (GOT and MDH B) and PCR amplification revealing spacer-size variation in the 5s-rDNA cluster were found most convenient. Specific characterisation of the two genomic components was possible using RFLP and in situ hybridisation. The strong molecular differentiation between S. officinarum and E. arundinaceus allows the identification of numerous Erianthus-specific RFLP bands in the hybrids. Genomic DNA in situ hybridisation allows for the differentiation of the chromosomes contributed by S. officinarum and E. arundinaceus in chromosome preparations of the hybrids. In situ hybridisation with the 18s-5.8s-25s rDNA probe highlights the basic chromosome numbers in the two parental species. The potential of these techniques to monitor the Erianthus genome during the introgression process is discussed.Type of Medium: Electronic ResourceURL: -
11Daugrois, J. H. ; Grivet, L. ; Roques, D. ; Hoarau, J. Y. ; Lombard, H. ; Glaszmann, J. C. ; D’Hont, A.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Key words Major gene ; Polyploidy ; Puccinia melanocephala ; Sugarcane ; RFLP ; RustSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Inheritance of resistance to rust was investigated in the self progeny of the sugarcane cultivar ‘R570’ also used to build a RFLP genetic map. Resistance was evaluated through both field and controlled greenhouse trials. A clear-cut 3 (resistant) : 1 (susceptible) segregation indicative of a probable dominant resistant gene was observed. This is the first documented report of a monogenic inheritance for disease resistance in sugarcane. This gene was found linked at 10 cM with an RFLP marker revealed by probe CDSR29. Other minor factors involved in the resistance were also detected.Type of Medium: Electronic ResourceURL: -
12Dufour, P. ; Deu, M. ; Grivet, L. ; D’Hont, A. ; Paulet, F. ; Bouet, A. ; Lanaud, C. ; Glaszmann, J. C. ; Hamon, P.
Springer
Published 1997Staff ViewISSN: 1432-2242Keywords: Key words Sorghum ; RFLP map ; Comparative mapping ; SugarcaneSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract A sorghum composite linkage map was constructed with two recombinant inbred line populations using heterologous probes already mapped on maize and sugarcane. This map includes 199 loci revealed by 188 probes and distributed on 13 linkage groups. A comparison based on 84 common probes was performed between the sorghum composite map and a map of a sugarcane (Saccharum spp.) cultivar being developed and presently comprising 10 tentative linkage groups. A straight synteny was observed for 2 pairs of linkage groups; in two cases, 1 sorghum linkage group corresponded to 2 or 3 sugarcane linkage groups, respectively; in two cases 1 sugarcane link- age group corresponded to 2 separate sorghum linkage groups; for 2 sorghum linkage groups, no complete correspondance was found in the sugarcane genome. In most cases loci appeared to be colinear between homoeologous chromosomal segments in sorghum and sugarcane. These results are discussed in relation to published data on sorghum genomic maps, with specific reference to the genetic organization of sugarcane cultivars, and they, illustrate how investigations on relatively simple diploid genomes as sorghum will facilitate the mapping of related polyploid species such as sugarcane.Type of Medium: Electronic ResourceURL: -
13Jannoo, N. ; Grivet, L. ; Seguin, M. ; Paulet, F. ; Domaingue, R. ; Rao, P. S. ; Dookun, A. ; D’Hont, A. ; Glaszmann, J. C.
Springer
Published 1999Staff ViewISSN: 1432-2242Keywords: Key words Saccharum officinarum ; Sugarcane cultivars ; Polyploid ; Genetic diversity ; RFLPSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Molecular diversity was analysed among 162 clones of sugarcane using DNA restriction fragment length polymorphism (RFLP). One hundred and nine of them were modern cultivars of interspecific origin; most of them were bred in Barbados or in Mauritius. Fifty three were from Saccharum officinarum species, which is the major source of genes in modern cultivars, prevailing over the part of the genome incorporated from the wild species Saccharum spontaneum. Twelve low-copy nuclear DNA probes scattered over the genome were used in combination with one or two restriction enzymes. A total of 399 fragments was identified, 386 of which were polymorphic. Each sugarcane clone displayed a high number of fragments per probe/enzyme combination, illustrating the polyploid constitution of the genome. Among the S. officinarum clones, those from New Guinea had the largest variability and encompassed that present among clones collected from the Indonesian Islands and those known to have been involved in the parentage of modern cultivars. This is in agreement with the hypothesis that New Guinea is the centre of origin of this species. The clones from New Caledonia formed a separate group and could correspond to S. officinarum clones modified through introgression with other members of the ‘Saccharum complex’. Despite the low number of S. officinarum clones used for breeding cultivars, more than 80% of the markers present in the whole S. officinarum sample were also found in modern cultivars due probably to a high heterozygosity related to polyploidy. Among the cultivars, the two main groups, originating from Barbados and Mauritius, were clearly separated. This appeared essentially due to S. spontaneum alleles present in Mauritian cultivars and absent in Barbadan ones, probably in relation to the regular use of early generation interspecific hybrids in the breeding program employed in Mauritius.Type of Medium: Electronic ResourceURL: -
14Dufour, P. ; Grivet, L. ; D'Hont, A. ; Deu, M. ; Trouche, G. ; Glaszmann, J. C. ; Hamon, P.
Springer
Published 1996Staff ViewISSN: 1432-2242Keywords: Maize ; Sorghum ; Sugarcane ; RFLP ; SyntenySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Comparative mapping within maize, sorghum and sugarcane has previously revealed the existence of syntenic regions between the crops. In the present study, mapping on the sorghum genome of a set of probes previously located on the maize and sugarcane maps allow a detailed analysis of the relationship between maize chromosomes 3 and 8 and sorghum and sugarcane homoeologous regions. Of 49 loci revealed by 46 (4 sugarcane and 42 maize) polymorphic probes in sorghum, 42 were linked and were assigned to linkage groups G (28), E (10) and I (4). On the basis of common probes, a complete co-linearity is observed between sorghum linkage group G and the two sugarcane linkage groups II and III. The comparison between the consensus sorghum/sugarcane map (G/II/III) and the maps of maize chromosomes 3 and 8 reveals a series of linkage blocks within which gene orders are conserved. These blocks are interspersed with non-homoeologous regions corresponding to the central part of the two maize chromosomes and have been reshuffled, resulting in several inversions in maize compared to sorghum and sugarcane. The results emphasize the fact that duplication will considerably complicate precise comparative mapping at the whole genome scale between maize and other Poaceae.Type of Medium: Electronic ResourceURL: -
15Barre, P. ; Layssac, M. ; D’Hont, A. ; Louarn, J. ; Charrier, A. ; Hamon, S. ; Noirot, M.
Springer
Published 1998Staff ViewISSN: 1432-2242Keywords: Key words Coffea ; Interspecific hybrids ; Genomic in situ hybridisation (GISH) ; Flow cytometrySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract F1 hybrids were obtained between two coffee species with the same chromosome number (2n=22) but with different nuclear DNA contents [C. pseudozanguebariae (PSE) 2C=1.13 pg and C. liberica var ‘dewevrei’ (DEW) 2C=1.42 pg]. G2 hybrids were obtained by open-pollination of the F1 hybrids. Genomic in situ hybridisation (GISH) and flow cytometry were used on six F1 hybrids and seven G2 hybrids to determine their parental chromosomic contribution and their nuclear DNA content (qDNA), respectively. GISH efficiently identified chromosomes from both species. F1 hybrids had a qDNA intermediate between that of the parental species and contained the expected 11 chromosomes from each species. There was a linear relationship between the number of PSE chromosomes and the nuclear DNA content, which indicates that flow cytometry can be used to give a rough estimate of the parental chromosomic contribution in G2 hybrids.Type of Medium: Electronic ResourceURL: -
16D'Hont, A. ; Grivet, L. ; Feldmann, P. ; Glaszmann, J. C. ; Rao, S. ; Berding, N.
Springer
Published 1996Staff ViewISSN: 1617-4623Keywords: Saccharum spp. ; In situ hybridization ; Polyploidy ; Intergenomic recombination ; rRNA genesSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Cultivated sugarcane clones (Saccharum spp., 2n=100 to 130) are derived from complex interspecific hybridizations between the speciesS. officinarum andS. spontaneum. Using comparative genomic DNA in situ hybridization, we demonstrated that it is possible to distinguish the chromosomes contributed by these two species in an interspecific F1 hybrid and a cultivated clone, R570. In the interspecific F1 studied, we observed n+n transmission of the parental chromosomes instead of the peculiar 2n+n transmission usually described in such crosses. Among the chromosomes of cultivar R570 (2n=107–115) about 10% were identified as originating fromS. spontaneum and about 10% were identified as recombinant chromosomes between the two speciesS. officinarum andS. spontaneum. This demonstrated for the first time the occurrence of recombination between the chromosomes of these two species. The rDNA sites were located by in situ hybridization in these two species and the cultivar R570. This supported different basic chromosome numbers and chromosome structural differences between the two species and provided a first bridge between physical and genetical mapping in sugarcane.Type of Medium: Electronic ResourceURL: -
17Lashermes, P. ; Combes, M.-C. ; Robert, J. ; Trouslot, P. ; D'Hont, A. ; Anthony, F. ; Charrier, A.
Springer
Published 1999Staff ViewISSN: 1617-4623Keywords: Key words Africa ; Amphidiploidy ; Coffee tree ; Molecular cytogenetics ; Restriction fragment length polymorphismSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Restriction fragment length polymorphism (RFLP) markers were used in combination with genomic in situ hybridisation (GISH) to investigate the origin of the allotetraploid species Coffea arabica (2n = 44). By comparing the RFLP patterns of potential diploid progenitor species with those of C. arabica, the sources of the two sets of chromosomes, or genomes, combined in C. arabica were identified. The genome organisation of C. arabica was confirmed by GISH using simultaneously labelled total genomic DNA from the two putative genome donor species as probes. These results clearly suggest that C. arabica is an amphidiploid formed by hybridisation between C. eugenioides and C. canephora, or ecotypes related to these diploid species. Our results also indicate low divergence between the two constituent genomes of C. arabica and those of its progenitor species, suggesting that the speciation of C. arabica took place relatively recently. Precise localisation in Central Africa of the site of the speciation of C. arabica, based on the present distribution of the coffee species, appears difficult, since the constitution and extent of tropical forest has varied considerably during the late Quaternary period.Type of Medium: Electronic ResourceURL: -
18Lu, Y. H. ; D'Hont, A. ; Walker, D. I. T. ; Rao, P. S. ; Feldmann, P. ; Glaszmann, J. C.
Springer
Published 1994Staff ViewISSN: 1573-5060Keywords: introgression ; phylogeny ; polyploidy ; RFLP ; Saccharum ; sugarcaneSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary DNA restriction fragment length polymorphism (RFLP) analysis was performed on 50 wild and old cultivated sugarcane accessions. Ninety-four maize low copy nuclear DNA sequences of known chromosomal position were screened for hybridization to digested sugarcane genomic DNA blots. Seventy-five (80%) gave very strong hybridization signals and usually yielded many bands and detected profuse polymorphism. Twenty-nine probes and 36 probe/enzyme combinations were selected on the basis of the scorability of the banding profiles. A total of 1110 fragments were separately identified among the 50 genotypes. Multivariate analyses of the data allowed the separation of the three basic species, Saccharum spontaneum, S. robustum and S. officinarum, showed that S. spontaneum had structure which could be related to the geographic origin of the clones and supported current hypotheses on the origin of secondary species S. barberi and S. sinense. The use of more probes did not improve the resolution between the various species examined but identified a few key polymorphisms which were not accounted for by current phylogenetic hypotheses and can guide future analyses. RFLPs in sugarcane will be useful essentially for depicting the genomic constitution of modern varieties of interspecific origin.Type of Medium: Electronic ResourceURL: -
19Glaszmann, J.C. ; Dufour, P. ; Grivet, L. ; D'Hont, A. ; Deu, M. ; Paulet, F. ; Hamon, P.
Springer
Published 1997Staff ViewISSN: 1573-5060Keywords: genome ; tropical grasses ; comparative mapping ; synteny ; collinearitySource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract The availability of molecular markers allowed comparing genetic maps between distinct taxa. Among the Poaceae (grasses) family, species as divergent as rice, wheat and maize show collinearity within wide regions along the whole genome. This opens the way for the integration of knowledge across all grasses and the development of molecular tools of general interest using the simple genome of rice. Many agronomically important tropical grasses belong to two major sub-families. Comparisons between maize, sorghum and sugarcane, three members of the Andropogoneae tribe, are briefly reviewed. One example of a particular genome region is used for extending the comparison to rice and illustrating various applications of comparative genome mapping.Type of Medium: Electronic ResourceURL: -
20Lu, Y. H. ; D'Hont, A. ; Paulet, F. ; Grivet, L. ; Arnaud, M. ; Glaszmann, J. C.
Springer
Published 1994Staff ViewISSN: 1573-5060Keywords: aneuploidy ; linkage disequilibrium ; polyploidy ; RFLP ; sugarcane varieties ; Saccharum officinarumSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary RFLP analysis was performed on 40 sugarcane cultivated varieties. Twenty-two maize low copy DNA clones located on different regions of the 10 maize chromosomes were used as probes to survey variability among the sugarcane varieties. A total of 425 fragments, 411 of which were polymorphic, were identified for 22 probe/enzyme combinations. Each variety displayed an average of 7.28 fragments per combination, revealing the complex polyploid origin of modern sugarcane varieties. The average genetic similarity between sugarcane varieties was 0.61. Although cultivated varieties appear closely related to S. officinarum clones, the genes of S. spontaneum seem to constitute the principal component of varietal diversity. A very weak global structuring among the 40 varieties is observed, in agreement with the profuse exchanges of parental materials between sugarcane breeding stations. Traces of linkage disequilibrium can be attributed to the distribution of S. spontaneum chromosomes among sugarcane varieties. The possibility of using modern varieties as a population for detecting associations between molecular markers and agronomic traits is suggested.Type of Medium: Electronic ResourceURL: